Expression profiles of the MXD3 gene and association of sequence variants with growth traits in Xianan and Qinchuan cattle

Dan Hao; Bo Thomsen; Jiangsong Bai; Shujun Peng; Xianyong Lan; Yongzhen Huang; Xiao Wang; Hong Chen

doi:10.1002/vms3.251

Expression profiles of the MXD3 gene and association of sequence variants with growth traits in Xianan and Qinchuan cattle

Dan Hao, Bo Thomsen, Jiangsong Bai, Shujun Peng, Xianyong Lan, Yongzhen Huang, Xiao Wang^*, Hong Chen

^*Corresponding author for this work

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Abstract

Max dimerization protein 3 (MXD3) belongs to the MYC superfamily of basic helix-loop-helix leucine zipper transcription factors, and MXD3-MAX heterodimers can bind to promoters of target genes to modulate their expression. The aim of this study was to determine the MXD3 mRNA expression levels in various cattle tissues comprising heart, liver, spleen, lung, kidney, Longissimus dorsi muscle and subcutaneous fat in Chinese Qinchuan and Xianan cattle breeds. The RT-qPCR data showed that the MXD3 gene was variably expressed between all tissues and at levels that were significantly different between two breeds (p T and g.3801 T>C) and one SNP in 3'untranslated region (3'UTR) (g.6263 G>A) of MXD3 gene. Association analysis revealed strong associations between pairwise and triple SNP combinations and the growth traits. Based on these results, we suggest that MXD3 polymorphisms could be useful as molecular markers in the Chinese beef cattle breeding program.

Original language	English
Journal	Veterinary Medicine and Science
Number of pages	11
ISSN	2053-1095
DOIs	https://doi.org/10.1002/vms3.251
Publication status	Published - 2020

Keywords

Cattle
Expression level
Growth trait
MXD3 gene

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title = "Expression profiles of the MXD3 gene and association of sequence variants with growth traits in Xianan and Qinchuan cattle",

abstract = "Max dimerization protein 3 (MXD3) belongs to the MYC superfamily of basic helix-loop-helix leucine zipper transcription factors, and MXD3-MAX heterodimers can bind to promoters of target genes to modulate their expression. The aim of this study was to determine the MXD3 mRNA expression levels in various cattle tissues comprising heart, liver, spleen, lung, kidney, Longissimus dorsi muscle and subcutaneous fat in Chinese Qinchuan and Xianan cattle breeds. The RT-qPCR data showed that the MXD3 gene was variably expressed between all tissues and at levels that were significantly different between two breeds (p T and g.3801 T>C) and one SNP in 3'untranslated region (3'UTR) (g.6263 G>A) of MXD3 gene. Association analysis revealed strong associations between pairwise and triple SNP combinations and the growth traits. Based on these results, we suggest that MXD3 polymorphisms could be useful as molecular markers in the Chinese beef cattle breeding program.",

keywords = "Cattle, Expression level, Growth trait, MXD3 gene",

author = "Dan Hao and Bo Thomsen and Jiangsong Bai and Shujun Peng and Xianyong Lan and Yongzhen Huang and Xiao Wang and Hong Chen",

year = "2020",

doi = "10.1002/vms3.251",

language = "English",

journal = "Veterinary Medicine and Science",

issn = "2053-1095",

publisher = "JohnWiley & Sons Ltd.",

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TY - JOUR

T1 - Expression profiles of the MXD3 gene and association of sequence variants with growth traits in Xianan and Qinchuan cattle

AU - Hao, Dan

AU - Thomsen, Bo

AU - Bai, Jiangsong

AU - Peng, Shujun

AU - Lan, Xianyong

AU - Huang, Yongzhen

AU - Wang, Xiao

AU - Chen, Hong

PY - 2020

Y1 - 2020

N2 - Max dimerization protein 3 (MXD3) belongs to the MYC superfamily of basic helix-loop-helix leucine zipper transcription factors, and MXD3-MAX heterodimers can bind to promoters of target genes to modulate their expression. The aim of this study was to determine the MXD3 mRNA expression levels in various cattle tissues comprising heart, liver, spleen, lung, kidney, Longissimus dorsi muscle and subcutaneous fat in Chinese Qinchuan and Xianan cattle breeds. The RT-qPCR data showed that the MXD3 gene was variably expressed between all tissues and at levels that were significantly different between two breeds (p T and g.3801 T>C) and one SNP in 3'untranslated region (3'UTR) (g.6263 G>A) of MXD3 gene. Association analysis revealed strong associations between pairwise and triple SNP combinations and the growth traits. Based on these results, we suggest that MXD3 polymorphisms could be useful as molecular markers in the Chinese beef cattle breeding program.

AB - Max dimerization protein 3 (MXD3) belongs to the MYC superfamily of basic helix-loop-helix leucine zipper transcription factors, and MXD3-MAX heterodimers can bind to promoters of target genes to modulate their expression. The aim of this study was to determine the MXD3 mRNA expression levels in various cattle tissues comprising heart, liver, spleen, lung, kidney, Longissimus dorsi muscle and subcutaneous fat in Chinese Qinchuan and Xianan cattle breeds. The RT-qPCR data showed that the MXD3 gene was variably expressed between all tissues and at levels that were significantly different between two breeds (p T and g.3801 T>C) and one SNP in 3'untranslated region (3'UTR) (g.6263 G>A) of MXD3 gene. Association analysis revealed strong associations between pairwise and triple SNP combinations and the growth traits. Based on these results, we suggest that MXD3 polymorphisms could be useful as molecular markers in the Chinese beef cattle breeding program.

KW - Cattle

KW - Expression level

KW - Growth trait

KW - MXD3 gene

U2 - 10.1002/vms3.251

DO - 10.1002/vms3.251

M3 - Journal article

C2 - 32141244

SN - 2053-1095

JO - Veterinary Medicine and Science

JF - Veterinary Medicine and Science

ER -

Expression profiles of the MXD3 gene and association of sequence variants with growth traits in Xianan and Qinchuan cattle

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Keywords

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