Max dimerization protein 3 (MXD3) belongs to the MYC superfamily of basic helix-loop-helix leucine zipper transcription factors, and MXD3-MAX heterodimers can bind to promoters of target genes to modulate their expression. The aim of this study was to determine the MXD3 mRNA expression levels in various cattle tissues comprising heart, liver, spleen, lung, kidney, Longissimus dorsi muscle and subcutaneous fat in Chinese Qinchuan and Xianan cattle breeds. The RT-qPCR data showed that the MXD3 gene was variably expressed between all tissues and at levels that were significantly different between two breeds (p T and g.3801 T>C) and one SNP in 3'untranslated region (3'UTR) (g.6263 G>A) of MXD3 gene. Association analysis revealed strong associations between pairwise and triple SNP combinations and the growth traits. Based on these results, we suggest that MXD3 polymorphisms could be useful as molecular markers in the Chinese beef cattle breeding program.
- Expression level
- Growth trait
- MXD3 gene
Hao, D., Thomsen, B., Bai, J., Peng, S., Lan, X., Huang, Y., Wang, X., & Chen, H. (2020). Expression profiles of the MXD3 gene and association of sequence variants with growth traits in Xianan and Qinchuan cattle. Veterinary Medicine and Science. https://doi.org/10.1002/vms3.251