Expression of the glycoprotein of viral haemorrhagic septicaemia virus (VHSV) on the surface of the fish cell line RTG-P1 induces type 1 interferon expression in neighbouring cells

F. Acosta, B. Collet, Niels Lorenzen, A.E. Ellis

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    In the present study using a luciferase/Mx promoter reporter system, it was shown that the rainbow trout gonad cell line (RTG-P1), a fibroblastic cell line, produces IFN when transfected with a plasmid encoding the glycoprotein of VHSV but not with plasmid vector alone. Only a small percentage of the cells expressed the G protein on the surface membrane as indicated by immunostaining of transfected cells. When transfection was performed in the presence of monoclonal antibodies (Mab) to the glycoprotein, the production of interferon mRNA transcripts was reduced by over 50%. This indicates that the surface expression of G protein was the major mechanism of interferon induction and that most of the interferon was being expressed by cells neighbouring the transfected cells. Crown
    Original languageEnglish
    JournalFish & shellfish immunology
    Volume21
    Issue number3
    Pages (from-to)272-278
    ISSN1050-4648
    DOIs
    Publication statusPublished - 2006

    Keywords

    • VHS virus
    • glycoprotein
    • rainbow trout cells
    • interferon

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