Abstract
Original language | English |
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Article number | 281 |
Journal | B M C Veterinary Research |
Volume | 14 |
Number of pages | 11 |
ISSN | 1746-6148 |
DOIs | |
Publication status | Published - 2018 |
Bibliographical note
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)Keywords
- Classical scrapie
- Innate immune response
- qPCR
- Whole blood
- Liver tissue
- Brain tissue
- Sheep
Cite this
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Expression of selected genes isolated from whole blood, liver and obex in lambs with experimental classical scrapie and healthy controls, showing a systemic innate immune response at the clinical end-stage. / Meling, Siv; Skovgaard, Kerstin; Bårdsen, Kjetil; Heegaard, Peter Mikael Helweg; Ulvund, Martha J.
In: B M C Veterinary Research, Vol. 14, 281, 2018.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Expression of selected genes isolated from whole blood, liver and obex in lambs with experimental classical scrapie and healthy controls, showing a systemic innate immune response at the clinical end-stage
AU - Meling, Siv
AU - Skovgaard, Kerstin
AU - Bårdsen, Kjetil
AU - Heegaard, Peter Mikael Helweg
AU - Ulvund, Martha J.
N1 - This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)
PY - 2018
Y1 - 2018
N2 - Background: Incubation period, disease progression, pathology and clinical presentation of classical scrapie in sheep are highly dependent on PRNP genotype, time and route of inoculation and prion strain. Our experimental model with pre-colostrum inoculation of homozygous VRQ lambs has shown to be an effective model with extensive PrPSc dissemination in lymphatic tissue and a short incubation period with severe clinical disease. Serum protein analysis has shown an elevation of acute phase proteins in the clinical stages of this experimental model, and here, we investigate changes in gene expression in whole blood, liver and brain. Results: The animals in the scrapie group showed severe signs of illness 22 weeks post inoculation necessitating euthanasia at 23 weeks post inoculation. This severe clinical presentation was accompanied by changes in expression of several genes. The following genes were differentially expressed in whole blood: TLR2, TLR4, C3, IL1B, LF and SAA, in liver tissue, the following genes differentially expressed: TNF-α, SAA, HP, CP, AAT, TTR and TF, and in the brain tissue, the following genes were differentially expressed: HP, CP, ALB and TTR. Conclusions: We report a strong and evident transcriptional innate immune response in the terminal stage of classical scrapie in these animals. The PRNP genotype and time of inoculation are believed to contribute to the clinical presentation, including the extensive dissemination of PrPSc throughout the lymphatic tissue.
AB - Background: Incubation period, disease progression, pathology and clinical presentation of classical scrapie in sheep are highly dependent on PRNP genotype, time and route of inoculation and prion strain. Our experimental model with pre-colostrum inoculation of homozygous VRQ lambs has shown to be an effective model with extensive PrPSc dissemination in lymphatic tissue and a short incubation period with severe clinical disease. Serum protein analysis has shown an elevation of acute phase proteins in the clinical stages of this experimental model, and here, we investigate changes in gene expression in whole blood, liver and brain. Results: The animals in the scrapie group showed severe signs of illness 22 weeks post inoculation necessitating euthanasia at 23 weeks post inoculation. This severe clinical presentation was accompanied by changes in expression of several genes. The following genes were differentially expressed in whole blood: TLR2, TLR4, C3, IL1B, LF and SAA, in liver tissue, the following genes differentially expressed: TNF-α, SAA, HP, CP, AAT, TTR and TF, and in the brain tissue, the following genes were differentially expressed: HP, CP, ALB and TTR. Conclusions: We report a strong and evident transcriptional innate immune response in the terminal stage of classical scrapie in these animals. The PRNP genotype and time of inoculation are believed to contribute to the clinical presentation, including the extensive dissemination of PrPSc throughout the lymphatic tissue.
KW - Classical scrapie
KW - Innate immune response
KW - qPCR
KW - Whole blood
KW - Liver tissue
KW - Brain tissue
KW - Sheep
U2 - 10.1186/s12917-018-1607-9
DO - 10.1186/s12917-018-1607-9
M3 - Journal article
VL - 14
JO - B M C Veterinary Research
JF - B M C Veterinary Research
SN - 1746-6148
M1 - 281
ER -