Expression of fungal biosynthetic gene clusters in S. cerevisiae for natural product discovery

Zihe Liu, Zhenquan Lin, Jens Nielsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

7 Downloads (Pure)

Abstract

Fungi are well known for production of antibiotics and other bioactive secondary metabolites, that can be served as pharmaceuticals, therapeutic agents and industrially useful compounds. However, compared with the characterization of prokaryotic biosynthetic gene clusters (BGCs), less attention has been paid to evaluate fungal BGCs. This is partially because heterologous expression of eukaryotic gene constructs often requires replacement of original promoters and terminators, as well as removal of intron sequences, and this substantially slow down the workflow in natural product discovery. It is therefore of interest to investigate the possibility and effectiveness of heterologous expression and library screening of intact BGCs without refactoring in industrial friendly microbial cell factories, such as the yeast Saccharomyces cerevisiae. Here, we discuss the importance of developing new research directions on library screening of fungal BGCs in yeast without refactoring, followed by outlooking prominent opportunities and challenges for future advancement.

Original languageEnglish
JournalSynthetic and Systems Biotechnology
Volume6
Issue number1
Pages (from-to)20-22
ISSN2405-805x
DOIs
Publication statusPublished - Mar 2021

Bibliographical note

Funding Information:
This work was supported by National Key Research and Development Program ( 2018YFA0901800 ), National Natural Science Foundation of China ( 22078012 ), Chinese Postdoctoral Science Foundation ( 2020M670115 ), the Novo Nordisk Foundation ( NNF10CC1016517 ), the Knut and Alice Wallenberg Foundation and Beijing Advanced Innovation Center for Soft Matter Science and Engineering .

Funding Information:
This work was supported by National Key Research and Development Program (2018YFA0901800), National Natural Science Foundation of China (22078012), Chinese Postdoctoral Science Foundation (2020M670115), the Novo Nordisk Foundation (NNF10CC1016517), the Knut and Alice Wallenberg Foundation and Beijing Advanced Innovation Center for Soft Matter Science and Engineering.

Publisher Copyright:
© 2021

Keywords

  • Cross-species engineering
  • High-throughput BGC cloning
  • Natural product discovery
  • Spliceosome
  • Synthetic biology
  • Yeast

Fingerprint

Dive into the research topics of 'Expression of fungal biosynthetic gene clusters in S. cerevisiae for natural product discovery'. Together they form a unique fingerprint.

Cite this