Expression of coding (mRNA) and non-coding (microRNA) RNA in lung tissue and blood isolated from pigs suffering from bacterial pleuropneumonia

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    MicroRNAs are small non-coding RNA molecules (18-23 nt), that regulate the activity of other genes at the post-transcriptional level. Recently it has become evident that microRNA plays an important role in modulating and fine tuning innate and adaptive immune responses. Still, little is known about the impact of microRNAs in the development and pathogenesis of lung infections. Expression of microRNA known to be induced by bacterial (i.e., LPS) ligands and thus supposed to play a role in the regulation of antimicrobial defence, were studied in lung tissue and in blood from pigs experimentally infected with Actinobacillus pleuropneumoniae (AP). Expression differences of mRNA and microRNA were quantified at different time points (6h, 12h, 24h, 48h PI) using reverse transcription quantitative real-time PCR (Rotor-Gene and Fluidigm). Expression profiles of miRNA in blood of seven animals were further studied using miRCURY™ LNA arrays (Exiqon, Denmark). All AP infected animals had significantly higher levels of mRNA coding for inflammatory mediators as IL-6 and IL-8 as well as the acute phase protein SAA, in the lung compared to the control group. MiR-223 was found to be highly up regulated, followed by miR-146a and to a lesser degree miR- 155 in lung tissue of the AP infected animals. MiR-233 was also found to be up regulated in blood based on both microarray and real-time PCR. Mir-233 has been found to be a negative regulator of neutrophil proliferation and activation, and might act to limit the potentially harmful consequences of the accumulation of infiltrating neutrophils in AP infected lungs.
    Original languageEnglish
    Title of host publicationGraduate School of Immunology 5th Annual Meeting : Translational Immunology: from vaccines to immunotherapy
    Number of pages72
    Publication date2010
    Publication statusPublished - 2010
    EventGraduate School of Immunology Annual Meeting - Rebild Bakker, Denmark
    Duration: 1 Jan 2010 → …
    Conference number: 5


    ConferenceGraduate School of Immunology Annual Meeting
    CityRebild Bakker, Denmark
    Period01/01/2010 → …


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