Exploring the Plant–Microbe Interface by Profiling the Surface-Associated Proteins of Barley Grains

Abida Sultan, Birgit Andersen, Birte Svensson, Christine Finnie

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Abstract

Cereal grains are colonized by a microbial community that actively interacts with the plant via secretion of various enzymes, hormones, and metabolites. Microorganisms decompose plant tissues by a collection of depolymerizing enzymes, including β-1,4-xylanases, that are in turn inhibited by plant xylanase inhibitors. To gain insight into the importance of the microbial consortia and their interaction with barley grains, we used a combined gel-based (2-DE coupled to MALDI-TOF-TOF MS) and gel-free (LC–MS/MS) proteomics approach complemented with enzyme activity assays to profile the surface-associated proteins and xylanolytic activities of two barley cultivars. The surface-associated proteome was dominated by plant proteins with roles in defense and stress-responses, while the relatively less abundant microbial (bacterial and fungal) proteins were involved in cell-wall and polysaccharide degradation and included xylanases. The surface-associated proteomes showed elevated xylanolytic activity and contained several xylanases. Integration of proteomics with enzyme assays is a powerful tool for analysis and characterization of the interaction between microbial consortia and plants in their natural environment.
Original languageEnglish
JournalJournal of Proteome Research
Volume15
Issue number4
Pages (from-to)1151-1167
Number of pages17
ISSN1535-3893
DOIs
Publication statusPublished - 2016

Keywords

  • Cereal proteins
  • Mass spectrometry
  • Proteome analysis
  • Xylanase activity
  • Metaproteome
  • Microbial communities
  • Environmental proteomics

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