Experimental Results and Integrated Modeling of Bacterial Growth on an Insoluble Hydrophobic Substrate (Phenanthrene)

Iris K. U. Adam, Arno Rein, Anja Miltner, Ana C. D. Fulgencio, Stefan Trapp, Matthias Kaestner

Research output: Contribution to journalJournal articleResearchpeer-review

380 Downloads (Pure)

Abstract

Metabolism of a low-solubility substrate is limited by dissolution and availability and can hardly be determined. We developed a numerical model for simultaneously calculating dissolution kinetics of such substrates and their metabolism and microbial growth (Monod kinetics with decay) and tested it with three aerobic phenanthrene (PHE) degraders: Novosphingobium pentaromativorans US6-1, Sphingomonas sp. EPA505, and Sphingobium yanoikuyae B1. PHE was present as microcrystals, providing non-limiting conditions for growth. Total PHE and protein concentration were tracked over 6-12 days. The model was fitted to the test results for the rates of dissolution, metabolism, and growth. The strains showed similar efficiency, with v(max) values of 12-18 g dw g(-1) d(-1), yields of 0.21 g g(-1), maximum growth rates of 2.5-3.8 d(-1), and decay rates of 0.04-0.05 d(-1). Sensitivity analysis with the model shows that (i) retention in crystals or NAPLs or by sequestration competes with biodegradation, (ii) bacterial growth conditions (dissolution flux and resulting chemical activity of substrate) are more relevant for the final state of the system than the initial biomass, and (iii) the desorption flux regulates the turnover in the presence of solid-state, sequestered (aged), or NAPL substrate sources.
Original languageEnglish
JournalEnvironmental Science & Technology (Washington)
Volume48
Issue number15
Pages (from-to)8717-8726
Number of pages10
ISSN0013-936X
DOIs
Publication statusPublished - 2014

Keywords

  • ENGINEERING,
  • ENVIRONMENTAL
  • POLYCYCLIC AROMATIC-HYDROCARBONS
  • MICROBIAL-DEGRADATION
  • ACTIVATED CARBON
  • BIODEGRADATION
  • SOIL
  • ANTHRACENE
  • KINETICS
  • CREOSOTE
  • BIOREMEDIATION
  • SPHINGOMONAS

Fingerprint Dive into the research topics of 'Experimental Results and Integrated Modeling of Bacterial Growth on an Insoluble Hydrophobic Substrate (Phenanthrene)'. Together they form a unique fingerprint.

Cite this