Abstract
Strains carrying deletions in the atp genes, encoding the H+-ATPase, were unable to grow on nonfermentable substrates such as succinate, whereas with glucose as the substrate the growth rate of an atp deletion mutant was surprisingly high (some 75-80% of wild-type growth rate). The rate of glucose and oxygen consumption of these mutants was increased compared to the wild-type rates. In order to analyze the importance of the H+-ATPase at its physiological level, the cellular concentration of H+-ATPase was modulated around the wild-type level, using genetically manipulated strains. The control coefficient by the H+-ATPase with respect to growth rate and catabolic fluxes was measured. Control on growth rate was absent at the wildtype concentration of H+-ATPase, independent of whether the substrate for growth was glucose or succinate. Control by the H+-ATPase on the catabolic fluxes, including respiration, was negative at the wild-type H+-ATPase level. Moreover, the turnover number of the individual H+-ATPase enzymes increased as the H+-ATPase concentration was lowered. The negative control by the H+-ATPase on catabolism may thus be involved in a homeostatic control of ATP synthesis and, to some extent, explain the zero control by the H+-ATPase on E. coli growth rate.
Original language | English |
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Journal | Journal of Bioenergetics and Biomembranes |
Volume | 27 |
Issue number | 6 |
Pages (from-to) | 543-554 |
Number of pages | 12 |
ISSN | 0145-479X |
DOIs | |
Publication status | Published - 1995 |
Keywords
- Escherichia coli
- Gene Deletion
- Genes, Bacterial
- Glucose
- Kinetics
- Operon
- Oxygen Consumption
- Proton-Translocating ATPases
- Succinates
- EC 3.6.3.14 Proton-Translocating ATPases
- IY9XDZ35W2 Glucose
- BIOPHYSICS
- CELL
- PROTON-TRANSLOCATING ATPASE
- NUCLEOTIDE-SEQUENCE
- OXIDATIVE-PHOSPHORYLATION
- ADENOSINE-TRIPHOSPHATASE
- SYNTHASE
- OPERON
- GENES
- PROMOTERS
- SUBUNIT
- MUTATIONS
- oxidative phosphorylation
- respiration
- ATP
- fluxes
- Metabolic Control Analysis
- Oxidative phosphorylation
- ATP GENE
- BACTERIAL GROWTH RATE
- CATABOLIC FLUX
- GENETICS
- MUTATION
- OXIDATIVE PHOSPHORYLATION
- OXYGEN CONSUMPTION
- RESPIRATION
- Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli
- ATP 56-65-5Q, 42530-29-0Q, 94587-45-8Q, 111839-44-2Q
- ATPASE 9000-83-3
- OXYGEN 7782-44-7
- 02506, Cytology - Animal
- 10012, Biochemistry - Gases
- 10060, Biochemistry studies - General
- 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
- 10510, Biophysics - Bioenergetics: electron transport and oxidative phosphorylation
- 10808, Enzymes - Physiological studies
- 13003, Metabolism - Energy and respiratory metabolism
- 16004, Respiratory system - Physiology and biochemistry
- 31000, Physiology and biochemistry of bacteria
- 31500, Genetics of bacteria and viruses
- 36002, Medical and clinical microbiology - Bacteriology
- Biochemistry and Molecular Biophysics
- Bioenergetics
- Cell Biology
- Enzymology
- Genetics
- Infection
- Metabolism
- Physiology