Experimental anal infection of rainbow trout with Flavobacterium psychrophilum : A novel challenge model

Jiwan Kumar Chettri, Azmi Al-Jubury, Inger Dalsgaard, Peter Mikael Helweg Heegaard, Kurt Buchmann*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Flavobacterium psychrophilum is a Gram‐negative psychrophilic bacterium causing rainbow trout fry syndrome (RTFS) in fry and bacterial coldwater disease (BCWD) in older fish. Both diseases challenge fish welfare and economy in hatcheries and in on‐growing facilities. The bacteria enter hosts through gills, skin, and the gastrointestinal tract, and transfer horizontally in contaminated water and vertically with sexual products of both male and female fish (Madetoja, Dalsgaard, & Wiklund, 2002; Madsen & Dalsgaard, 1999; Nematollahi, Decostere, Pasmans, & Haesebrouck, 2003). Protection afforded by experimental vaccination (injection or immersion) using bacterins (formalin‐killed whole cell) has been described (Hoare, Ngo, Bartie, & Adams, 2017; Madetoja et al., 2006), although no commercial vaccine is presently available for control of RTFS and BCWD. Further research on RTFS/BCWD vaccinology will benefit from an improved challenge method as current methods comprising intraperitoneal (i.p.) injection, bath, and bath exposure after treatment with stressors such as hydrogen peroxide (Henriksen, Kania, Buchmann, & Dalsgaard, 2015; Madsen & Dalsgaard, 1999) remain difficult to reproduce and rely on wounding the structural integrity of mucosal surfaces. The present study compares different infection methods and evaluates systems where the rainbow trout surface (skin, gills, and gut) is kept intact or injured. We compared six different challenge methods comprising anal intubation, i.p. injection, co‐habitation, and bath challenge exposing either nontreated intact fish, fish chemically damaged by exposure to hydrogen peroxide or fish mechanically damaged by needle insertion in the tail‐fin. Disease development was subsequently recorded for 4 weeks.
Original languageEnglish
JournalJournal of Fish Diseases
Volume41
Issue number12
Pages (from-to)1917-1919
Number of pages3
ISSN0140-7775
DOIs
Publication statusPublished - 2018

Keywords

  • Challenge model
  • Bacterial Cold Water Disease BCWD
  • Flavobacterium
  • Rainbow trout
  • Rainbow trout fry syndrome

Cite this

@article{97b2faff3590431da15e8240bbeed48d,
title = "Experimental anal infection of rainbow trout with Flavobacterium psychrophilum : A novel challenge model",
abstract = "Flavobacterium psychrophilum is a Gram‐negative psychrophilic bacterium causing rainbow trout fry syndrome (RTFS) in fry and bacterial coldwater disease (BCWD) in older fish. Both diseases challenge fish welfare and economy in hatcheries and in on‐growing facilities. The bacteria enter hosts through gills, skin, and the gastrointestinal tract, and transfer horizontally in contaminated water and vertically with sexual products of both male and female fish (Madetoja, Dalsgaard, & Wiklund, 2002; Madsen & Dalsgaard, 1999; Nematollahi, Decostere, Pasmans, & Haesebrouck, 2003). Protection afforded by experimental vaccination (injection or immersion) using bacterins (formalin‐killed whole cell) has been described (Hoare, Ngo, Bartie, & Adams, 2017; Madetoja et al., 2006), although no commercial vaccine is presently available for control of RTFS and BCWD. Further research on RTFS/BCWD vaccinology will benefit from an improved challenge method as current methods comprising intraperitoneal (i.p.) injection, bath, and bath exposure after treatment with stressors such as hydrogen peroxide (Henriksen, Kania, Buchmann, & Dalsgaard, 2015; Madsen & Dalsgaard, 1999) remain difficult to reproduce and rely on wounding the structural integrity of mucosal surfaces. The present study compares different infection methods and evaluates systems where the rainbow trout surface (skin, gills, and gut) is kept intact or injured. We compared six different challenge methods comprising anal intubation, i.p. injection, co‐habitation, and bath challenge exposing either nontreated intact fish, fish chemically damaged by exposure to hydrogen peroxide or fish mechanically damaged by needle insertion in the tail‐fin. Disease development was subsequently recorded for 4 weeks.",
keywords = "Challenge model, Bacterial Cold Water Disease BCWD, Flavobacterium, Rainbow trout, Rainbow trout fry syndrome",
author = "Chettri, {Jiwan Kumar} and Azmi Al-Jubury and Inger Dalsgaard and Heegaard, {Peter Mikael Helweg} and Kurt Buchmann",
year = "2018",
doi = "10.1111/jfd.12888",
language = "English",
volume = "41",
pages = "1917--1919",
journal = "Journal of Fish Diseases",
issn = "0140-7775",
publisher = "Wiley-Blackwell",
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}

Experimental anal infection of rainbow trout with Flavobacterium psychrophilum : A novel challenge model. / Chettri, Jiwan Kumar; Al-Jubury, Azmi; Dalsgaard, Inger; Heegaard, Peter Mikael Helweg; Buchmann, Kurt.

In: Journal of Fish Diseases, Vol. 41, No. 12, 2018, p. 1917-1919.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Experimental anal infection of rainbow trout with Flavobacterium psychrophilum : A novel challenge model

AU - Chettri, Jiwan Kumar

AU - Al-Jubury, Azmi

AU - Dalsgaard, Inger

AU - Heegaard, Peter Mikael Helweg

AU - Buchmann, Kurt

PY - 2018

Y1 - 2018

N2 - Flavobacterium psychrophilum is a Gram‐negative psychrophilic bacterium causing rainbow trout fry syndrome (RTFS) in fry and bacterial coldwater disease (BCWD) in older fish. Both diseases challenge fish welfare and economy in hatcheries and in on‐growing facilities. The bacteria enter hosts through gills, skin, and the gastrointestinal tract, and transfer horizontally in contaminated water and vertically with sexual products of both male and female fish (Madetoja, Dalsgaard, & Wiklund, 2002; Madsen & Dalsgaard, 1999; Nematollahi, Decostere, Pasmans, & Haesebrouck, 2003). Protection afforded by experimental vaccination (injection or immersion) using bacterins (formalin‐killed whole cell) has been described (Hoare, Ngo, Bartie, & Adams, 2017; Madetoja et al., 2006), although no commercial vaccine is presently available for control of RTFS and BCWD. Further research on RTFS/BCWD vaccinology will benefit from an improved challenge method as current methods comprising intraperitoneal (i.p.) injection, bath, and bath exposure after treatment with stressors such as hydrogen peroxide (Henriksen, Kania, Buchmann, & Dalsgaard, 2015; Madsen & Dalsgaard, 1999) remain difficult to reproduce and rely on wounding the structural integrity of mucosal surfaces. The present study compares different infection methods and evaluates systems where the rainbow trout surface (skin, gills, and gut) is kept intact or injured. We compared six different challenge methods comprising anal intubation, i.p. injection, co‐habitation, and bath challenge exposing either nontreated intact fish, fish chemically damaged by exposure to hydrogen peroxide or fish mechanically damaged by needle insertion in the tail‐fin. Disease development was subsequently recorded for 4 weeks.

AB - Flavobacterium psychrophilum is a Gram‐negative psychrophilic bacterium causing rainbow trout fry syndrome (RTFS) in fry and bacterial coldwater disease (BCWD) in older fish. Both diseases challenge fish welfare and economy in hatcheries and in on‐growing facilities. The bacteria enter hosts through gills, skin, and the gastrointestinal tract, and transfer horizontally in contaminated water and vertically with sexual products of both male and female fish (Madetoja, Dalsgaard, & Wiklund, 2002; Madsen & Dalsgaard, 1999; Nematollahi, Decostere, Pasmans, & Haesebrouck, 2003). Protection afforded by experimental vaccination (injection or immersion) using bacterins (formalin‐killed whole cell) has been described (Hoare, Ngo, Bartie, & Adams, 2017; Madetoja et al., 2006), although no commercial vaccine is presently available for control of RTFS and BCWD. Further research on RTFS/BCWD vaccinology will benefit from an improved challenge method as current methods comprising intraperitoneal (i.p.) injection, bath, and bath exposure after treatment with stressors such as hydrogen peroxide (Henriksen, Kania, Buchmann, & Dalsgaard, 2015; Madsen & Dalsgaard, 1999) remain difficult to reproduce and rely on wounding the structural integrity of mucosal surfaces. The present study compares different infection methods and evaluates systems where the rainbow trout surface (skin, gills, and gut) is kept intact or injured. We compared six different challenge methods comprising anal intubation, i.p. injection, co‐habitation, and bath challenge exposing either nontreated intact fish, fish chemically damaged by exposure to hydrogen peroxide or fish mechanically damaged by needle insertion in the tail‐fin. Disease development was subsequently recorded for 4 weeks.

KW - Challenge model

KW - Bacterial Cold Water Disease BCWD

KW - Flavobacterium

KW - Rainbow trout

KW - Rainbow trout fry syndrome

U2 - 10.1111/jfd.12888

DO - 10.1111/jfd.12888

M3 - Journal article

VL - 41

SP - 1917

EP - 1919

JO - Journal of Fish Diseases

JF - Journal of Fish Diseases

SN - 0140-7775

IS - 12

ER -