Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine

Lasse E. Pedersen, Jared R Patch, Mary Kenney, Raisa A Glabman, Morten Nielsen, Gregers Jungersen, Søren Buus, William T Golde

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The immune response to the highly acute foot-and-mouth disease virus (FMDV) is routinely reported as a measure of serum antibody. However, a critical effector function of immune responses combating viral infection of mammals is the cytotoxic T lymphocyte (CTL) response mediated by virus specific CD8 expressing T cells. This immune mechanism arrests viral spread by killing virus infected cells before new, mature virus can develop. We have previously shown that infection of swine by FMDV results in a measurable CTL response and have correlated CTL killing of virus-infected cells with specific class I major histocompatibility complex (MHC) tetramer staining. We also showed that a modified replication defective human adenovirus 5 vector expressing the FMDV structural proteins (Ad5-FMDV-T vaccine) targets the induction of a CD8(+) CTL response with a minimal humoral response. In this report, we show that the specificity of the CD8(+) T cell response to Ad5-FMDV-T varies between cohorts of genetically identical animals. Further, we demonstrate epitope specificity of CD8(+) T cells expands following multiple immunizations with this vaccine.
    Original languageEnglish
    JournalVeterinary Immunology and Immunopathology
    Volume181
    Pages (from-to)59-67
    Number of pages9
    ISSN0165-2427
    DOIs
    Publication statusPublished - 2016

    Keywords

    • Class I
    • Cytotoxic T lymphocytes
    • MHC
    • MHC tetramers
    • SLA
    • T cell epitopes

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