Exo- and surface proteomes of the probiotic bacterium Lactobacillus acidophilus NCFM

Hasan Ufuk Celebioglu, Birte Svensson

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Lactobacillus acidophilus NCFM is a well-known probiotic bacterium extensively studied for its beneficial health effects. Exoproteome (proteins exported into culture medium) and surface proteome (proteins attached to S-layer) of this probiotic were identified by using 2DE followed by MALDI TOF MS to find proteins potentially involved in bacteria-host interactions. The exo- and surface proteomes included 43 and 39 different proteins from 72 and 49 successfully identified spots, respectively. Twenty-two proteins were shared between the two proteomes; both contained the major surface layer protein that participates in host interaction as well as several well-known and putative moonlighting proteins. The exoproteome contained 9 classically-secreted (containing a signal sequence) and 10 non-classically secreted proteins, while the surface proteome contained four classically-secreted and 8 non-classically secreted proteins. Identification of exo- and surface proteomes contributes describing potential protein-mediated probiotic-host interactions.
Original languageEnglish
Article number1700019
Issue number11
Number of pages16
Publication statusPublished - 2017


  • Moonlighting proteins
  • Probiotics
  • Secreted proteins
  • Subproteome
  • Surface proteins


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