Examining the Potential of Plasma-Assisted Pretreated Wheat Straw for Enzyme Production by Trichoderma reesei

Divanery Rodríguez Gómez, Linda Olkjær Lehmann, Nadja Schultz-Jensen, Anne Belinda Bjerre, Timothy John Hobley

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Plasma-assisted pretreated wheat straw was investigated for cellulase and xylanase production by Trichoderma reesei fermentation. Fermentations were conducted with media containing washed and unwashed plasma-assisted pretreated wheat straw as carbon source which was sterilized by autoclavation. To account for any effects of autoclavation, a comparison was made with unsterilized media containing antibiotics. It was found that unsterilized washed plasma-assisted pretreated wheat straw (which contained antibiotics) was best suited for the production of xylanases (110 IU ml(-1)) and cellulases (0.5 filter paper units (FPU) ml(-1)). Addition of Avicel boosted enzyme titers with the highest cellulase titers (1.5 FPU ml(-1)) found with addition of 50 % w/w Avicel and with the highest xylanase production (350 IU ml(-1)) reached in the presence of 10 % w/w Avicel. Comparison with enzyme titers from other nonrefined feedstocks suggests that plasma pretreated wheat straw is a promising and suitable substrate for cellulase and hemicellulase production.
Original languageEnglish
JournalApplied Biochemistry and Biotechnology
Issue number8
Pages (from-to)2051-2063
Publication statusPublished - 2012


  • plasma-assisted pretreated wheat straw
  • synergistic effect
  • Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi Imperfecti or Deuteromycetes [15500] Trichoderma reesei species fermentation agent strain-Rut-C30
  • Avicel boosted enzyme
  • cellulase 9012-54-8 EC
  • hemicellulase 9025-56-3
  • xylanase 37278-89-0 EC
  • 10060, Biochemistry studies - General
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 39008, Food microbiology - General and miscellaneous
  • 51522, Plant physiology - Chemical constituents
  • autoclavation laboratory techniques
  • Biochemistry and Molecular Biophysics
  • Bioprocess Engineering


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