Ex vivo intestinal perfusion model for investigating mucoadhesion of microcontainers

Mette Dalskov Mosgaard*, Sophie Strindberg, Zarmeena Abid, Ritika Singh Petersen, Lasse Højlund Eklund Thamdrup, Alina Joukainen Andersen, Stephan Sylvest Keller, Anette Müllertz, Line Hagner Nielsen, Anja Boisen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Micro fabricated delivery systems have shown promise in increasing oral bioavailability of drugs. Micrometer-sized polymeric devices (microcontainers) have the potential to facilitate unidirectional drug release directly into the intestinal mucosa whereby, drug absorption can be enhanced. The aim of this study was to develop an ex vivo model to investigate mucosal adhesion and orientation of microcontainers. Furthermore, to investigate how microcontainers with varying height, shape and material behave in regards to mucoadhesion and orientation. Microcontainers were placed at the top of an inclined piece of porcine small intestine. The tissue was perfused with biorelevant medium followed by microscopic examination to observe the orientation and amount of microcontainers on the tissue. The mucoadhesion of the microcontainers were evaluated based on the observed position on the tissue after being exposed to flow. When comparing the varying types of microcontainers, good adhesion was in general observed since most of the microcontainers were located in the beginning of the intestine. Microcontainers fabricated from the epoxy-based photoresist SU-8 had a slightly better adherence than those fabricated from poly-ɛ-caprolactone (PCL). The orientation of the microcontainers appeare to be dictated mainly by the height. In general, the model showed promising results in evaluating mucoadhesion and orientation.
Original languageEnglish
Article number118658
JournalINTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume570
Number of pages8
ISSN0378-5173
DOIs
Publication statusPublished - 2019

Keywords

  • Microdevices
  • Microcontainers
  • Oral drug delivery
  • Ex vivo intestinal perfusion
  • Mucoadhesion

Cite this

@article{adc957a86e1e469fba124a07f93504d5,
title = "Ex vivo intestinal perfusion model for investigating mucoadhesion of microcontainers",
abstract = "Micro fabricated delivery systems have shown promise in increasing oral bioavailability of drugs. Micrometer-sized polymeric devices (microcontainers) have the potential to facilitate unidirectional drug release directly into the intestinal mucosa whereby, drug absorption can be enhanced. The aim of this study was to develop an ex vivo model to investigate mucosal adhesion and orientation of microcontainers. Furthermore, to investigate how microcontainers with varying height, shape and material behave in regards to mucoadhesion and orientation. Microcontainers were placed at the top of an inclined piece of porcine small intestine. The tissue was perfused with biorelevant medium followed by microscopic examination to observe the orientation and amount of microcontainers on the tissue. The mucoadhesion of the microcontainers were evaluated based on the observed position on the tissue after being exposed to flow. When comparing the varying types of microcontainers, good adhesion was in general observed since most of the microcontainers were located in the beginning of the intestine. Microcontainers fabricated from the epoxy-based photoresist SU-8 had a slightly better adherence than those fabricated from poly-ɛ-caprolactone (PCL). The orientation of the microcontainers appeare to be dictated mainly by the height. In general, the model showed promising results in evaluating mucoadhesion and orientation.",
keywords = "Microdevices, Microcontainers, Oral drug delivery, Ex vivo intestinal perfusion, Mucoadhesion",
author = "Mosgaard, {Mette Dalskov} and Sophie Strindberg and Zarmeena Abid and Petersen, {Ritika Singh} and Thamdrup, {Lasse H{\o}jlund Eklund} and Andersen, {Alina Joukainen} and Keller, {Stephan Sylvest} and Anette M{\"u}llertz and Nielsen, {Line Hagner} and Anja Boisen",
year = "2019",
doi = "10.1016/j.ijpharm.2019.118658",
language = "English",
volume = "570",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier",

}

Ex vivo intestinal perfusion model for investigating mucoadhesion of microcontainers. / Mosgaard, Mette Dalskov; Strindberg, Sophie; Abid, Zarmeena; Petersen, Ritika Singh; Thamdrup, Lasse Højlund Eklund; Andersen, Alina Joukainen; Keller, Stephan Sylvest; Müllertz, Anette; Nielsen, Line Hagner; Boisen, Anja.

In: INTERNATIONAL JOURNAL OF PHARMACEUTICS, Vol. 570, 118658, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Ex vivo intestinal perfusion model for investigating mucoadhesion of microcontainers

AU - Mosgaard, Mette Dalskov

AU - Strindberg, Sophie

AU - Abid, Zarmeena

AU - Petersen, Ritika Singh

AU - Thamdrup, Lasse Højlund Eklund

AU - Andersen, Alina Joukainen

AU - Keller, Stephan Sylvest

AU - Müllertz, Anette

AU - Nielsen, Line Hagner

AU - Boisen, Anja

PY - 2019

Y1 - 2019

N2 - Micro fabricated delivery systems have shown promise in increasing oral bioavailability of drugs. Micrometer-sized polymeric devices (microcontainers) have the potential to facilitate unidirectional drug release directly into the intestinal mucosa whereby, drug absorption can be enhanced. The aim of this study was to develop an ex vivo model to investigate mucosal adhesion and orientation of microcontainers. Furthermore, to investigate how microcontainers with varying height, shape and material behave in regards to mucoadhesion and orientation. Microcontainers were placed at the top of an inclined piece of porcine small intestine. The tissue was perfused with biorelevant medium followed by microscopic examination to observe the orientation and amount of microcontainers on the tissue. The mucoadhesion of the microcontainers were evaluated based on the observed position on the tissue after being exposed to flow. When comparing the varying types of microcontainers, good adhesion was in general observed since most of the microcontainers were located in the beginning of the intestine. Microcontainers fabricated from the epoxy-based photoresist SU-8 had a slightly better adherence than those fabricated from poly-ɛ-caprolactone (PCL). The orientation of the microcontainers appeare to be dictated mainly by the height. In general, the model showed promising results in evaluating mucoadhesion and orientation.

AB - Micro fabricated delivery systems have shown promise in increasing oral bioavailability of drugs. Micrometer-sized polymeric devices (microcontainers) have the potential to facilitate unidirectional drug release directly into the intestinal mucosa whereby, drug absorption can be enhanced. The aim of this study was to develop an ex vivo model to investigate mucosal adhesion and orientation of microcontainers. Furthermore, to investigate how microcontainers with varying height, shape and material behave in regards to mucoadhesion and orientation. Microcontainers were placed at the top of an inclined piece of porcine small intestine. The tissue was perfused with biorelevant medium followed by microscopic examination to observe the orientation and amount of microcontainers on the tissue. The mucoadhesion of the microcontainers were evaluated based on the observed position on the tissue after being exposed to flow. When comparing the varying types of microcontainers, good adhesion was in general observed since most of the microcontainers were located in the beginning of the intestine. Microcontainers fabricated from the epoxy-based photoresist SU-8 had a slightly better adherence than those fabricated from poly-ɛ-caprolactone (PCL). The orientation of the microcontainers appeare to be dictated mainly by the height. In general, the model showed promising results in evaluating mucoadhesion and orientation.

KW - Microdevices

KW - Microcontainers

KW - Oral drug delivery

KW - Ex vivo intestinal perfusion

KW - Mucoadhesion

U2 - 10.1016/j.ijpharm.2019.118658

DO - 10.1016/j.ijpharm.2019.118658

M3 - Journal article

VL - 570

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

M1 - 118658

ER -