TY - JOUR
T1 - Evolutionary highways to persistent bacterial infection
AU - Bartell, Jennifer
AU - Sommer, Lea M.
AU - Haagensen, Janus Anders Juul
AU - Loch, Anne
AU - Espinosa Portero, Rocio
AU - Molin, Søren
AU - Johansen, Helle Krogh
PY - 2019
Y1 - 2019
N2 -
Persistent infections require bacteria to evolve from their naive colonization state by optimizing fitness in the host via simultaneous adaptation of multiple traits, which can obscure evolutionary trends and complicate infection management. Accordingly, here we screen 8 infection-relevant phenotypes of 443 longitudinal Pseudomonas aeruginosa isolates from 39 young cystic fibrosis patients over 10 years. Using statistical modeling, we map evolutionary trajectories and identify trait correlations accounting for patient-specific influences. By integrating previous genetic analyses of 474 isolates, we provide a window into early adaptation to the host, finding: (1) a 2-3 year timeline of rapid adaptation after colonization, (2) variant "naive" and "adapted" states reflecting discordance between phenotypic and genetic adaptation, (3) adaptive trajectories leading to persistent infection via three distinct evolutionary modes, and (4) new associations between phenotypes and pathoadaptive mutations. Ultimately, we effectively deconvolute complex trait adaptation, offering a framework for evolutionary studies and precision medicine in clinical microbiology.
AB -
Persistent infections require bacteria to evolve from their naive colonization state by optimizing fitness in the host via simultaneous adaptation of multiple traits, which can obscure evolutionary trends and complicate infection management. Accordingly, here we screen 8 infection-relevant phenotypes of 443 longitudinal Pseudomonas aeruginosa isolates from 39 young cystic fibrosis patients over 10 years. Using statistical modeling, we map evolutionary trajectories and identify trait correlations accounting for patient-specific influences. By integrating previous genetic analyses of 474 isolates, we provide a window into early adaptation to the host, finding: (1) a 2-3 year timeline of rapid adaptation after colonization, (2) variant "naive" and "adapted" states reflecting discordance between phenotypic and genetic adaptation, (3) adaptive trajectories leading to persistent infection via three distinct evolutionary modes, and (4) new associations between phenotypes and pathoadaptive mutations. Ultimately, we effectively deconvolute complex trait adaptation, offering a framework for evolutionary studies and precision medicine in clinical microbiology.
KW - Evolutionary genetics
KW - Evolvability
KW - Microbial ecology
KW - Pathogens
U2 - 10.1038/s41467-019-08504-7
DO - 10.1038/s41467-019-08504-7
M3 - Journal article
C2 - 30733448
SN - 2041-1723
VL - 10
JO - Nature Communications
JF - Nature Communications
M1 - 629
ER -