Evolution and diversification of Pseudomonas aeruginosa in the paranasal sinuses of cystic fibrosis children have implications for chronic lung infection

Susse Kirkelund Hansen, Martin Holm Rau, Helle Krogh Johansen, Oana Ciofu, Lars Jelsbak, Lei Yang, Anders Folkesson, Hanne Østergaard Jarmer, Kasper Aanæs, Christian von Buchwald, Niels Høiby, Søren Molin

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The opportunistic pathogen Pseudomonas aeruginosa is a frequent colonizer of the airways of patients suffering from cystic fibrosis (CF). Depending on early treatment regimens, the colonization will, with high probability, develop into chronic infections sooner or later, and it is important to establish under which conditions the switch to chronic infection takes place. In association with a recently established sinus surgery treatment program for CF patients at the Copenhagen CF Center, colonization of the paranasal sinuses with P. aeruginosa has been investigated, paralleled by sampling of sputum from the same patients. On the basis of genotyping and phenotypic characterization including transcription profiling, the diversity of the P. aeruginosa populations in the sinuses and the lower airways was investigated and compared. The observations made from several children show that the paranasal sinuses constitute an important niche for the colonizing bacteria in many patients. The paranasal sinuses often harbor distinct bacterial subpopulations, and in the early colonization phases there seems to be a migration from the sinuses to the lower airways, suggesting that independent adaptation and evolution take place in the sinuses. Importantly, before the onset of chronic lung infection, lineages with mutations conferring a large fitness benefit in CF airways such as mucA and lasR as well as small colony variants and antibiotic-resistant clones are part of the sinus populations. Thus, the paranasal sinuses potentially constitute a protected niche of adapted clones of P. aeruginosa, which can intermittently seed the lungs and pave the way for subsequent chronic lung infections.
    Original languageEnglish
    JournalI S M E Journal
    Volume6
    Issue number1
    Pages (from-to)31
    ISSN1751-7362
    DOIs
    Publication statusPublished - 2012

    Cite this

    Hansen, Susse Kirkelund ; Rau, Martin Holm ; Johansen, Helle Krogh ; Ciofu, Oana ; Jelsbak, Lars ; Yang, Lei ; Folkesson, Anders ; Jarmer, Hanne Østergaard ; Aanæs, Kasper ; von Buchwald, Christian ; Høiby, Niels ; Molin, Søren. / Evolution and diversification of Pseudomonas aeruginosa in the paranasal sinuses of cystic fibrosis children have implications for chronic lung infection. In: I S M E Journal. 2012 ; Vol. 6, No. 1. pp. 31.
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    title = "Evolution and diversification of Pseudomonas aeruginosa in the paranasal sinuses of cystic fibrosis children have implications for chronic lung infection",
    abstract = "The opportunistic pathogen Pseudomonas aeruginosa is a frequent colonizer of the airways of patients suffering from cystic fibrosis (CF). Depending on early treatment regimens, the colonization will, with high probability, develop into chronic infections sooner or later, and it is important to establish under which conditions the switch to chronic infection takes place. In association with a recently established sinus surgery treatment program for CF patients at the Copenhagen CF Center, colonization of the paranasal sinuses with P. aeruginosa has been investigated, paralleled by sampling of sputum from the same patients. On the basis of genotyping and phenotypic characterization including transcription profiling, the diversity of the P. aeruginosa populations in the sinuses and the lower airways was investigated and compared. The observations made from several children show that the paranasal sinuses constitute an important niche for the colonizing bacteria in many patients. The paranasal sinuses often harbor distinct bacterial subpopulations, and in the early colonization phases there seems to be a migration from the sinuses to the lower airways, suggesting that independent adaptation and evolution take place in the sinuses. Importantly, before the onset of chronic lung infection, lineages with mutations conferring a large fitness benefit in CF airways such as mucA and lasR as well as small colony variants and antibiotic-resistant clones are part of the sinus populations. Thus, the paranasal sinuses potentially constitute a protected niche of adapted clones of P. aeruginosa, which can intermittently seed the lungs and pave the way for subsequent chronic lung infections.",
    author = "Hansen, {Susse Kirkelund} and Rau, {Martin Holm} and Johansen, {Helle Krogh} and Oana Ciofu and Lars Jelsbak and Lei Yang and Anders Folkesson and Jarmer, {Hanne {\O}stergaard} and Kasper Aan{\ae}s and {von Buchwald}, Christian and Niels H{\o}iby and S{\o}ren Molin",
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    doi = "10.1038/ismej.2011.83",
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    journal = "I S M E Journal",
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    Evolution and diversification of Pseudomonas aeruginosa in the paranasal sinuses of cystic fibrosis children have implications for chronic lung infection. / Hansen, Susse Kirkelund; Rau, Martin Holm; Johansen, Helle Krogh; Ciofu, Oana; Jelsbak, Lars; Yang, Lei; Folkesson, Anders; Jarmer, Hanne Østergaard; Aanæs, Kasper; von Buchwald, Christian; Høiby, Niels; Molin, Søren.

    In: I S M E Journal, Vol. 6, No. 1, 2012, p. 31.

    Research output: Contribution to journalJournal articleResearchpeer-review

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    T1 - Evolution and diversification of Pseudomonas aeruginosa in the paranasal sinuses of cystic fibrosis children have implications for chronic lung infection

    AU - Hansen, Susse Kirkelund

    AU - Rau, Martin Holm

    AU - Johansen, Helle Krogh

    AU - Ciofu, Oana

    AU - Jelsbak, Lars

    AU - Yang, Lei

    AU - Folkesson, Anders

    AU - Jarmer, Hanne Østergaard

    AU - Aanæs, Kasper

    AU - von Buchwald, Christian

    AU - Høiby, Niels

    AU - Molin, Søren

    PY - 2012

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    N2 - The opportunistic pathogen Pseudomonas aeruginosa is a frequent colonizer of the airways of patients suffering from cystic fibrosis (CF). Depending on early treatment regimens, the colonization will, with high probability, develop into chronic infections sooner or later, and it is important to establish under which conditions the switch to chronic infection takes place. In association with a recently established sinus surgery treatment program for CF patients at the Copenhagen CF Center, colonization of the paranasal sinuses with P. aeruginosa has been investigated, paralleled by sampling of sputum from the same patients. On the basis of genotyping and phenotypic characterization including transcription profiling, the diversity of the P. aeruginosa populations in the sinuses and the lower airways was investigated and compared. The observations made from several children show that the paranasal sinuses constitute an important niche for the colonizing bacteria in many patients. The paranasal sinuses often harbor distinct bacterial subpopulations, and in the early colonization phases there seems to be a migration from the sinuses to the lower airways, suggesting that independent adaptation and evolution take place in the sinuses. Importantly, before the onset of chronic lung infection, lineages with mutations conferring a large fitness benefit in CF airways such as mucA and lasR as well as small colony variants and antibiotic-resistant clones are part of the sinus populations. Thus, the paranasal sinuses potentially constitute a protected niche of adapted clones of P. aeruginosa, which can intermittently seed the lungs and pave the way for subsequent chronic lung infections.

    AB - The opportunistic pathogen Pseudomonas aeruginosa is a frequent colonizer of the airways of patients suffering from cystic fibrosis (CF). Depending on early treatment regimens, the colonization will, with high probability, develop into chronic infections sooner or later, and it is important to establish under which conditions the switch to chronic infection takes place. In association with a recently established sinus surgery treatment program for CF patients at the Copenhagen CF Center, colonization of the paranasal sinuses with P. aeruginosa has been investigated, paralleled by sampling of sputum from the same patients. On the basis of genotyping and phenotypic characterization including transcription profiling, the diversity of the P. aeruginosa populations in the sinuses and the lower airways was investigated and compared. The observations made from several children show that the paranasal sinuses constitute an important niche for the colonizing bacteria in many patients. The paranasal sinuses often harbor distinct bacterial subpopulations, and in the early colonization phases there seems to be a migration from the sinuses to the lower airways, suggesting that independent adaptation and evolution take place in the sinuses. Importantly, before the onset of chronic lung infection, lineages with mutations conferring a large fitness benefit in CF airways such as mucA and lasR as well as small colony variants and antibiotic-resistant clones are part of the sinus populations. Thus, the paranasal sinuses potentially constitute a protected niche of adapted clones of P. aeruginosa, which can intermittently seed the lungs and pave the way for subsequent chronic lung infections.

    U2 - 10.1038/ismej.2011.83

    DO - 10.1038/ismej.2011.83

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    VL - 6

    SP - 31

    JO - I S M E Journal

    JF - I S M E Journal

    SN - 1751-7362

    IS - 1

    ER -