Evidence for at least 4 antigenic sites on type-O foot-and-mouth-disease virus involved in virus neutralization - identification by single and multiple site monoclonal antibody-resistant mutants

D. McCahon, J. R. Crowther, Graham Belsham, J. D. A. Kitson, M. Duchesne, P. Have, R. H. Meloen, D. O. Morgan, F. de Simone

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Neutralizing monoclonal antibodies raised against type O foot-and-mouth disease virus have been characterized on the basis of their reactivity with a panel of single site monoclonal antibody-resistant mutants which had defined three antigenic sites. Five antibodies neutralized all these mutants, but by selecting further single site mutants with one of these antibodies it was possible to define a fourth site involved in virus neutralization. Two monoclonal antibodies still neutralized these mutants and all multiple site resistant mutants. One multiple site resistant mutant was resistant to neutralization at each of four antigenic sites but was still efficiently neutralized by type O convalescent cattle sera. The relationship between sites recognized by different monoclonal antibodies generated in different laboratories is discussed.
Original languageEnglish
JournalJournal of General Virology
Volume70
Issue numberPart 3
Pages (from-to)639-645
ISSN0022-1317
DOIs
Publication statusPublished - 1989
Externally publishedYes

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