Evaluation of the effects of spray drying parameters for producing cubosome powder precursors

Christoffer von Halling Laier*, Tommy Sonne Alstrøm, Mia Travers Bargholz, Pernille Bjerg Sjøltov, Thomas Rades, Anja Boisen, Line Hagner Nielsen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Vaccines provide great benefit for global health, but are insufficiently distributed in developing countries due to high costs of manufacturing and limited storage stability. Spray drying formulations of peptide-based vaccines offer a promising strategy to reduce production costs and improve unrefrigerated storage stability. This design of experiments investigated how adjusting spray drying parameters (inlet temperature, atomization pressure, feed rate and aspiration rate) affects residual moisture and reconstitution properties of the powder product, and morphology and size of the rehydrated particles. An emulsion capable of forming cubosomes with the protein ovalbumin as model antigen was used as vaccine formulation and spray dried. The produced powders had low residual moisture content, independent of the spray drying parameters within the range investigated. Cryogenic transmission electron microscopy revealed that cubosomes were produced after rehydration regardless of the spray drying parameters. Furthermore, dynamic light scattering showed that the average diameter of the rehydrated particles consistently was close to 230 nm at any combination of spray drying parameters as long as the outlet temperature was kept below a threshold of 115oC. The process thus appears to be robust allowing optimization to be focused on yield, dry particle engineering or outlet temperature considerations.
Original languageEnglish
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Volume135
Pages (from-to)44-48
ISSN0939-6411
DOIs
Publication statusPublished - 2018

Keywords

  • Ovalbumin
  • Particles
  • Colloids
  • Monoolein
  • Vaccine
  • Design of Experiments

Cite this

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title = "Evaluation of the effects of spray drying parameters for producing cubosome powder precursors",
abstract = "Vaccines provide great benefit for global health, but are insufficiently distributed in developing countries due to high costs of manufacturing and limited storage stability. Spray drying formulations of peptide-based vaccines offer a promising strategy to reduce production costs and improve unrefrigerated storage stability. This design of experiments investigated how adjusting spray drying parameters (inlet temperature, atomization pressure, feed rate and aspiration rate) affects residual moisture and reconstitution properties of the powder product, and morphology and size of the rehydrated particles. An emulsion capable of forming cubosomes with the protein ovalbumin as model antigen was used as vaccine formulation and spray dried. The produced powders had low residual moisture content, independent of the spray drying parameters within the range investigated. Cryogenic transmission electron microscopy revealed that cubosomes were produced after rehydration regardless of the spray drying parameters. Furthermore, dynamic light scattering showed that the average diameter of the rehydrated particles consistently was close to 230 nm at any combination of spray drying parameters as long as the outlet temperature was kept below a threshold of 115oC. The process thus appears to be robust allowing optimization to be focused on yield, dry particle engineering or outlet temperature considerations.",
keywords = "Ovalbumin, Particles, Colloids, Monoolein, Vaccine, Design of Experiments",
author = "{von Halling Laier}, Christoffer and Alstr{\o}m, {Tommy Sonne} and Bargholz, {Mia Travers} and Sj{\o}ltov, {Pernille Bjerg} and Thomas Rades and Anja Boisen and Nielsen, {Line Hagner}",
year = "2018",
doi = "10.1016/j.ejpb.2018.12.008",
language = "English",
volume = "135",
pages = "44--48",
journal = "European Journal of Pharmaceutics and Biopharmaceutics",
issn = "0939-6411",
publisher = "Elsevier",

}

Evaluation of the effects of spray drying parameters for producing cubosome powder precursors. / von Halling Laier, Christoffer; Alstrøm, Tommy Sonne; Bargholz, Mia Travers ; Sjøltov, Pernille Bjerg ; Rades, Thomas; Boisen, Anja; Nielsen, Line Hagner.

In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 135, 2018, p. 44-48.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Evaluation of the effects of spray drying parameters for producing cubosome powder precursors

AU - von Halling Laier, Christoffer

AU - Alstrøm, Tommy Sonne

AU - Bargholz, Mia Travers

AU - Sjøltov, Pernille Bjerg

AU - Rades, Thomas

AU - Boisen, Anja

AU - Nielsen, Line Hagner

PY - 2018

Y1 - 2018

N2 - Vaccines provide great benefit for global health, but are insufficiently distributed in developing countries due to high costs of manufacturing and limited storage stability. Spray drying formulations of peptide-based vaccines offer a promising strategy to reduce production costs and improve unrefrigerated storage stability. This design of experiments investigated how adjusting spray drying parameters (inlet temperature, atomization pressure, feed rate and aspiration rate) affects residual moisture and reconstitution properties of the powder product, and morphology and size of the rehydrated particles. An emulsion capable of forming cubosomes with the protein ovalbumin as model antigen was used as vaccine formulation and spray dried. The produced powders had low residual moisture content, independent of the spray drying parameters within the range investigated. Cryogenic transmission electron microscopy revealed that cubosomes were produced after rehydration regardless of the spray drying parameters. Furthermore, dynamic light scattering showed that the average diameter of the rehydrated particles consistently was close to 230 nm at any combination of spray drying parameters as long as the outlet temperature was kept below a threshold of 115oC. The process thus appears to be robust allowing optimization to be focused on yield, dry particle engineering or outlet temperature considerations.

AB - Vaccines provide great benefit for global health, but are insufficiently distributed in developing countries due to high costs of manufacturing and limited storage stability. Spray drying formulations of peptide-based vaccines offer a promising strategy to reduce production costs and improve unrefrigerated storage stability. This design of experiments investigated how adjusting spray drying parameters (inlet temperature, atomization pressure, feed rate and aspiration rate) affects residual moisture and reconstitution properties of the powder product, and morphology and size of the rehydrated particles. An emulsion capable of forming cubosomes with the protein ovalbumin as model antigen was used as vaccine formulation and spray dried. The produced powders had low residual moisture content, independent of the spray drying parameters within the range investigated. Cryogenic transmission electron microscopy revealed that cubosomes were produced after rehydration regardless of the spray drying parameters. Furthermore, dynamic light scattering showed that the average diameter of the rehydrated particles consistently was close to 230 nm at any combination of spray drying parameters as long as the outlet temperature was kept below a threshold of 115oC. The process thus appears to be robust allowing optimization to be focused on yield, dry particle engineering or outlet temperature considerations.

KW - Ovalbumin

KW - Particles

KW - Colloids

KW - Monoolein

KW - Vaccine

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