Evaluation of PCR for detection of Campylobacter in a national broiler surveillance programme in Denmark

Marianne Lund; A. Wedderkopp; M. Wainø; Steen Nordentoft; Dang Duong Bang; Karl Pedersen; Mogens Madsen

doi:10.1046/j.1365-2672.2003.01934.x

Evaluation of PCR for detection of Campylobacter in a national broiler surveillance programme in Denmark

Marianne Lund, A. Wedderkopp, M. Wainø, Steen Nordentoft, Dang Duong Bang, Karl Pedersen, Mogens Madsen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Aims: To develop and evaluate a rapid and sensitive PCR method for detection of Campylobacter spp. directly from chicken faeces. Methods and Results: DNA was isolated from faecal swabs using magnetic beads followed by PCR using a prealiquoted PCR mixture, which had been stored in the freezer. The result could be obtained in

Original language	English
Journal	Journal of Applied Microbiology
Volume	94
Issue number	5
Pages (from-to)	929-935
ISSN	1364-5072
DOIs	https://doi.org/10.1046/j.1365-2672.2003.01934.x
Publication status	Published - 2003

Keywords

DNA isolation
broiler
Campylobacter
PCR

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title = "Evaluation of PCR for detection of Campylobacter in a national broiler surveillance programme in Denmark",

abstract = "Aims: To develop and evaluate a rapid and sensitive PCR method for detection of Campylobacter spp. directly from chicken faeces. Methods and Results: DNA was isolated from faecal swabs using magnetic beads followed by PCR using a prealiquoted PCR mixture, which had been stored in the freezer. The result could be obtained in ",

keywords = "DNA isolation, broiler, Campylobacter, PCR",

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AU - Lund, Marianne

AU - Wedderkopp, A.

AU - Wainø, M.

AU - Nordentoft, Steen

AU - Bang, Dang Duong

AU - Pedersen, Karl

AU - Madsen, Mogens

PY - 2003

Y1 - 2003

N2 - Aims: To develop and evaluate a rapid and sensitive PCR method for detection of Campylobacter spp. directly from chicken faeces. Methods and Results: DNA was isolated from faecal swabs using magnetic beads followed by PCR using a prealiquoted PCR mixture, which had been stored in the freezer. The result could be obtained in

AB - Aims: To develop and evaluate a rapid and sensitive PCR method for detection of Campylobacter spp. directly from chicken faeces. Methods and Results: DNA was isolated from faecal swabs using magnetic beads followed by PCR using a prealiquoted PCR mixture, which had been stored in the freezer. The result could be obtained in

KW - DNA isolation

KW - broiler

KW - Campylobacter

KW - PCR

U2 - 10.1046/j.1365-2672.2003.01934.x

DO - 10.1046/j.1365-2672.2003.01934.x

M3 - Journal article

SN - 1364-5072

VL - 94

SP - 929

EP - 935

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

IS - 5

ER -

Evaluation of PCR for detection of Campylobacter in a national broiler surveillance programme in Denmark

Abstract

Keywords

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