Evaluation of immunofluorescence microscopy and enzyme-linked immunosorbent assay in detection of Cryptosporidium and Giardia infections in asymptomatic dogs

R. Rimhanen-Finne, Heidi L. Enemark, J. Kolehmainen, P. Toropainen, M.L. Hanninen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The performance of immunofluorescence microscopy (IF) and enzyme-linked immunosorbent assay (ELISA) in canine feces was evaluated. IF and Cryptosporidium ELISA detected 10(5) oocysts/g, while the detection limit for Giardia ELISA was 10(4) cysts/g. The Cryptosporidium ELISA showed 94% specificity but only 71% sensitivity. The Giardia ELISA correlated well with IF (sensitivity 100%, specificity 96%) and was capable of detecting animal specific Giardia duodenalis genotypes. Visual interpretation appeared appropriate for assessment of ELISA results. The proportion of positive samples and possible zoonotic character of Cryptosporidium and Giardia infections in 150 asymptomatic Finnish dogs from the Helsinki area were studied. The overall proportion of dogs positive for Cryptosporidium was 5% (7/150) and that for Giardia 5% (8/150). In dogs
    Original languageEnglish
    JournalVeterinary Parasitology
    Volume145
    Issue number3-4
    Pages (from-to)345-348
    ISSN0304-4017
    DOIs
    Publication statusPublished - 2007

    Keywords

    • Giardia
    • Cryptosporidium
    • ELISA
    • dog
    • IF

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