Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production

Jane Lindedam, Sander Bruun, Henning Jørgensen, Stephen R. Decker, Geoffrey B. Turner, Jaclyn D. DeMartini, Charles E. Wyman, Claus Felby

Research output: Contribution to journalJournal articleResearchpeer-review


We present a unique evaluation of three advanced high throughput pretreatment and enzymatic hydrolysis systems (HTPH-systems) for screening of lignocellulosic biomass for enzymatic saccharification. Straw from 20 cultivars of winter wheat from two sites in Denmark was hydrothermally pretreated and enzymatically processed in each of the separately engineered HTPH-systems at 1) University of California, Riverside, 2) National Renewable Energy Laboratory (NREL), Colorado, and 3) University of Copenhagen (CPH). All three systems were able to detect significant differences between the cultivars in the release of fermentable sugars, with average cellulose conversions of 57%, 64%, and 71% from Riverside, NREL and CPH, respectively. The best correlation of glucose yields was found between the Riverside and NREL systems (R-2 = 0.2139), and the best correlation for xylose yields was found between Riverside and CPH (R-2 = 0.4269). All three systems identified Flair as the highest yielding cultivar and Dinosor, Glasgow, and Robigus as low yielding cultivars. Despite different conditions in the three HTPH-systems, the approach of microscale screening for phenotypically less recalcitrant feedstock seems sufficiently robust to be used as a generic analytical platform. (C) 2014 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalBiomass & Bioenergy
Pages (from-to)261-267
Publication statusPublished - 2014


  • Denmark Europe Palearctic region
  • enzymatic saccharification
  • lignocellulosic biomass
  • recalcitrant feedstock
  • Monocotyledones Angiospermae Spermatophyta Plantae (Angiosperms, Monocots, Plants, Spermatophytes, Vascular Plants) - Gramineae [25305] winter wheat common cultivar-Flair, cultivar-Penso, cultivar-Abika, cultivar-Oakley, cultivar-Inspiration, cultivar-Audi, cultivar-Skalmeje, cultivar-Jenga, cultivar-Potenzial, cultivar-Glasgow, cultivar-Dinosor, cultivar-Smuggler, cultivar-Ambition, cultivar-Hattrick, cultivar-Florett, cultivar-Tommi, cultivar-Samyl, cultivar-Tuscan, cultivar-Robigus, cultivar-Opus
  • cellulose 9004-34-6
  • ethanol 64-17-5
  • fermentable sugars
  • glucose 58367-01-4
  • xylose 25990-60-7
  • 00512, General biology - Conservation and resource management
  • 04500, Mathematical biology and statistical methods
  • 10060, Biochemistry studies - General
  • 10068, Biochemistry studies - Carbohydrates
  • 39008, Food microbiology - General and miscellaneous
  • 51522, Plant physiology - Chemical constituents
  • Computational Biology
  • advanced high throughput pretreatment system laboratory techniques
  • enzymatic hydrolysis system laboratory techniques
  • high throughput screening method laboratory techniques
  • statistical analysis mathematical and computer techniques
  • Biochemistry and Molecular Biophysics
  • Bioprocess Engineering
  • Conservation
  • Mathematical Biology
  • High throughput screening
  • Lignocellulosic ethanol
  • Wheat straw
  • Genetic selection
  • Microscale pretreatment
  • Enzymatic hydrolysis
  • Lignocellulosic ethanols
  • Pre-Treatment
  • Wheat straws
  • Cellulosic ethanol


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