Evaluation of culture methods for rapid screening of swine faecal samples for Yersinia enterocolitica O : 3 biotype 4

Jeffrey Hoorfar, C.B.F. Holmvig

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In two studies, seven different culture protocols were compared to test naturally contaminated faecal samples from pigs for isolation of Y. enterocolitica serotype O; 3/biotype 4( n = 70 and n = 79). Four of the protocols were based on the Nordic Committee on Food Analysis (NMKL protocols), while three protocols were based on a rapid and selective method (here called ITC protocols). The protocols differed mainly in time of pre-enrichment (1, 10 and 24 d) and enrichment (2, 10, 24 d) and the type of selective enrichment media (ITC vs. MRB). The sensitivity of the rapid ITC protocol (24% and 9%) was comparable with the lengthy NMKL-protocols (16% and 11%), while the results of direct plating after 3 h (4%) and the extended enrichment in ITC-broth (4%) were very low. In addition, there was a marked reduction in the number of false positive plates in the short selective protocol (62% vs. 12%). The results indicate possibilities of shortening the culture methods by replacing most of the biochemical tests with an agglutination test based on a monoclonal antibody.
Original languageEnglish
JournalJournal of Veterinary Medicine Series B-Infectious Diseases and Veterinary Public Health
Volume46
Issue number3
Pages (from-to)189-198
ISSN0931-1793
Publication statusPublished - 1999

Cite this

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title = "Evaluation of culture methods for rapid screening of swine faecal samples for Yersinia enterocolitica O : 3 biotype 4",
abstract = "In two studies, seven different culture protocols were compared to test naturally contaminated faecal samples from pigs for isolation of Y. enterocolitica serotype O; 3/biotype 4( n = 70 and n = 79). Four of the protocols were based on the Nordic Committee on Food Analysis (NMKL protocols), while three protocols were based on a rapid and selective method (here called ITC protocols). The protocols differed mainly in time of pre-enrichment (1, 10 and 24 d) and enrichment (2, 10, 24 d) and the type of selective enrichment media (ITC vs. MRB). The sensitivity of the rapid ITC protocol (24{\%} and 9{\%}) was comparable with the lengthy NMKL-protocols (16{\%} and 11{\%}), while the results of direct plating after 3 h (4{\%}) and the extended enrichment in ITC-broth (4{\%}) were very low. In addition, there was a marked reduction in the number of false positive plates in the short selective protocol (62{\%} vs. 12{\%}). The results indicate possibilities of shortening the culture methods by replacing most of the biochemical tests with an agglutination test based on a monoclonal antibody.",
author = "Jeffrey Hoorfar and C.B.F. Holmvig",
year = "1999",
language = "English",
volume = "46",
pages = "189--198",
journal = "Journal of Veterinary Medicine Series B-Infectious Diseases and Veterinary Public Health",
issn = "0931-1793",
publisher = "Blackwell Publishing",
number = "3",

}

Evaluation of culture methods for rapid screening of swine faecal samples for Yersinia enterocolitica O : 3 biotype 4. / Hoorfar, Jeffrey; Holmvig, C.B.F.

In: Journal of Veterinary Medicine Series B-Infectious Diseases and Veterinary Public Health, Vol. 46, No. 3, 1999, p. 189-198.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Evaluation of culture methods for rapid screening of swine faecal samples for Yersinia enterocolitica O : 3 biotype 4

AU - Hoorfar, Jeffrey

AU - Holmvig, C.B.F.

PY - 1999

Y1 - 1999

N2 - In two studies, seven different culture protocols were compared to test naturally contaminated faecal samples from pigs for isolation of Y. enterocolitica serotype O; 3/biotype 4( n = 70 and n = 79). Four of the protocols were based on the Nordic Committee on Food Analysis (NMKL protocols), while three protocols were based on a rapid and selective method (here called ITC protocols). The protocols differed mainly in time of pre-enrichment (1, 10 and 24 d) and enrichment (2, 10, 24 d) and the type of selective enrichment media (ITC vs. MRB). The sensitivity of the rapid ITC protocol (24% and 9%) was comparable with the lengthy NMKL-protocols (16% and 11%), while the results of direct plating after 3 h (4%) and the extended enrichment in ITC-broth (4%) were very low. In addition, there was a marked reduction in the number of false positive plates in the short selective protocol (62% vs. 12%). The results indicate possibilities of shortening the culture methods by replacing most of the biochemical tests with an agglutination test based on a monoclonal antibody.

AB - In two studies, seven different culture protocols were compared to test naturally contaminated faecal samples from pigs for isolation of Y. enterocolitica serotype O; 3/biotype 4( n = 70 and n = 79). Four of the protocols were based on the Nordic Committee on Food Analysis (NMKL protocols), while three protocols were based on a rapid and selective method (here called ITC protocols). The protocols differed mainly in time of pre-enrichment (1, 10 and 24 d) and enrichment (2, 10, 24 d) and the type of selective enrichment media (ITC vs. MRB). The sensitivity of the rapid ITC protocol (24% and 9%) was comparable with the lengthy NMKL-protocols (16% and 11%), while the results of direct plating after 3 h (4%) and the extended enrichment in ITC-broth (4%) were very low. In addition, there was a marked reduction in the number of false positive plates in the short selective protocol (62% vs. 12%). The results indicate possibilities of shortening the culture methods by replacing most of the biochemical tests with an agglutination test based on a monoclonal antibody.

M3 - Journal article

VL - 46

SP - 189

EP - 198

JO - Journal of Veterinary Medicine Series B-Infectious Diseases and Veterinary Public Health

JF - Journal of Veterinary Medicine Series B-Infectious Diseases and Veterinary Public Health

SN - 0931-1793

IS - 3

ER -