Evaluation of Consolidated Bioprocessing of Sugarcane Biomass by a Multiple Hydrolytic Enzyme Producer Saccharomyces Yeast

Caroline L. Perez, Thais S. Milessi*, Juliana P. Sandri, Marcio D.N. Ramos, Bruna T. Carvalho, Arne Claes, Mekonnen M. Demeke, Johan M. Thevelein, Teresa C. Zangirolami

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Overcoming key bottlenecks for 2G ethanol production, such as the most suitable biomass pretreatment, process layout, and appropriate microorganism, is crucial to achieve a technically and economically feasible technology. The consolidated bioprocessing (CBP) is an approach that embodies one single step of enzyme production, biomass hydrolysis, and sugars fermentation. In this work, the CBP using S. cerevisiae AC14, a recombinant strain capable of secreting 7 different lignocellulosic enzymes and ferment xylose and glucose, was investigated in synthetic medium (cellobiose, corncob xylan, glucose, and xylose) and in industrial medium consisting of the solid fraction of hydrothermally pretreated sugarcane bagasse and its liquor. Results obtained were promising, with ethanol productivity of 4.46 g/L/h and complete hydrolysis of cellobiose and corncob xylan. In real industrial medium, 1.86 g/L/h of ethanol was reached, with partial conversion of both solid and liquid fractions. Further strategies to enhance enzymes access to complex substrates can improve process feasibility. Graphical Abstract: [Figure not available: see fulltext.]
Original languageEnglish
JournalBioenergy Research
Volume16
Pages (from-to)1973-1989
ISSN1939-1234
DOIs
Publication statusPublished - 2024

Keywords

  • Consolidated bioprocessing
  • Sugarcane biomass
  • Enzymes
  • Hydrothermal pretreatment
  • Recombinant Saccharomyces cerevisiae

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