Evaluation of air samplers and filter materials for collection and recovery of airborne norovirus

Katrine Uhrbrand*, Ismo Kalevi Koponen, Anna Charlotte Schultz, Anne Mette Madsen

*Corresponding author for this work

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Abstract

The aim of this study was to identify the most efficient sampling method for quantitative PCR-based detection of airborne human norovirus (NoV). Methods and Results: A comparative experiment was conducted in an aerosol chamber using aerosolised murine norovirus (MNV) as a surrogate for NoV. Sampling was performed using a nylon (NY) filter in conjunction with four kinds of personal samplers; Gesamtstaubprobenahme sampler (GSP), Triplex-cyclone sampler (TC), 3-piece closed-faced Millipore cassette (3P) and a 2-stage NIOSH cyclone sampler (NIO). In addition, sampling was performed using the GSP sampler with four different filter types; NY, polycarbonate (PC), polytetrafluoroethylene (PTFE) and gelatine (GEL). The sampling efficiency of MNV was significantly influenced by both sampler and filter type. The GSP sampler was found to give significantly (P<0.05) higher recovery of aerosolised MNV than 3P and NIO. A higher recovery was also found for GSP compared with TC, albeit not significantly. Finally, recovery of aerosolised MNV was significantly (P<0.05) higher using NY than PC, PTFE and GEL filters. Conclusions: The GSP sampler combined with a nylon filter was found to be the best method for personal filter-based sampling of airborne NoV. Significance and Impact of the Study. The identification of a suitable NoV air sampler is an important step towards studying the association between exposure to airborne NoV and infection.
Original languageEnglish
JournalJournal of Applied Microbiology
Volume124
Issue number4
Pages (from-to)990-1000
ISSN1364-5072
DOIs
Publication statusPublished - 2018

Keywords

  • Air sampling
  • Norovirus
  • Bioaerosol
  • Aerosolisation
  • GSP sampler
  • Filters
  • Airborne exposure

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