Evaluation of a commercial competitive enzyme-linked immunosorbent assay for detection of avian influenza virus subtype H5 antibodies in zoo birds

Trine Hammer Jensen*, Jannie Holmegaard Andersen, Charlotte Kristiane Hjulsager, Mariann Chriél, Mads Frost Bertelsen

*Corresponding author for this work

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The hemagglutination inhibition (HI) test is the current gold standard for detecting antibodies to avian influenza virus (AIV). Enzyme-linked immunosorbent assays (ELISAs) have been explored for use in poultry and certain wild bird species because of high efficiency and lower cost. This study compared a commercial ELISA for detection of AIV subtype H5 antibodies with HI test of 572 serum samples from zoo birds. There was no significant difference between the results of the two tests when statistically compared by a McNemar χ2 test (P = 0.86) and assessment of κ (κ = 0.87). With a specificity of 94.2% (95% confidence interval [CI], 0.92-0.97), a sensitivity of 93.9% (95% CI, 0.91-0.97), and an excellent correlation between the two tests, this ELISA can be recommended as an alternative to the HI test for preliminary screening of zoo bird sera for antibodies to AIV subtype H5.
    Original languageEnglish
    JournalJournal of Zoo and Wildlife Medicine
    Volume48
    Issue number3
    Pages (from-to)882-885
    ISSN1042-7260
    DOIs
    Publication statusPublished - 2017

    Keywords

    • Avian influenza
    • ELISA
    • H5
    • Hemagglutination inhibition test
    • Zoo birds

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