Evaluation of a blocking ELISA for screening of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus

K.J. Sørensen; Anette Bøtner; E.S. Madsen; Bertel Strandbygaard; Jens Nielsen

Evaluation of a blocking ELISA for screening of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus

K.J. Sørensen, Anette Bøtner, E.S. Madsen, Bertel Strandbygaard, Jens Nielsen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A blocking Elisa was developed for the detection of antibodies against PRRS virus with a view to satisfying the need for examination of blood samples on a large scale. The test was evaluated in comparison with an indirect Elisa and the immunoperoxidase monolayer assay. The blocking Elisa was sensitive and specific. It had a higher capacity and was cheaper to perform than the immunoperoxidase monolayer assay and the indirect Elisa. It was comparable to the immunoperoxidase monolayer assay and better than the indirect Elisa in detecting antibodies formed early after infection, and it was superior to both the immunoperoxidase monolayer assay and the indirect Elisa in detecting antibodies at a late stage of infection.

Original language	English
Journal	Veterinary Microbiology
Volume	56
Issue number	1-2
Pages (from-to)	1-8
ISSN	0378-1135
Publication status	Published - 1997

Keywords

PRRS diagnosis virus
antibodies
porcine reproductive and respiratory syndrome virus
ELISA

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title = "Evaluation of a blocking ELISA for screening of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus",

abstract = "A blocking Elisa was developed for the detection of antibodies against PRRS virus with a view to satisfying the need for examination of blood samples on a large scale. The test was evaluated in comparison with an indirect Elisa and the immunoperoxidase monolayer assay. The blocking Elisa was sensitive and specific. It had a higher capacity and was cheaper to perform than the immunoperoxidase monolayer assay and the indirect Elisa. It was comparable to the immunoperoxidase monolayer assay and better than the indirect Elisa in detecting antibodies formed early after infection, and it was superior to both the immunoperoxidase monolayer assay and the indirect Elisa in detecting antibodies at a late stage of infection.",

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year = "1997",

language = "English",

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journal = "Veterinary Microbiology",

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TY - JOUR

T1 - Evaluation of a blocking ELISA for screening of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus

AU - Sørensen, K.J.

AU - Bøtner, Anette

AU - Madsen, E.S.

AU - Strandbygaard, Bertel

AU - Nielsen, Jens

PY - 1997

Y1 - 1997

N2 - A blocking Elisa was developed for the detection of antibodies against PRRS virus with a view to satisfying the need for examination of blood samples on a large scale. The test was evaluated in comparison with an indirect Elisa and the immunoperoxidase monolayer assay. The blocking Elisa was sensitive and specific. It had a higher capacity and was cheaper to perform than the immunoperoxidase monolayer assay and the indirect Elisa. It was comparable to the immunoperoxidase monolayer assay and better than the indirect Elisa in detecting antibodies formed early after infection, and it was superior to both the immunoperoxidase monolayer assay and the indirect Elisa in detecting antibodies at a late stage of infection.

AB - A blocking Elisa was developed for the detection of antibodies against PRRS virus with a view to satisfying the need for examination of blood samples on a large scale. The test was evaluated in comparison with an indirect Elisa and the immunoperoxidase monolayer assay. The blocking Elisa was sensitive and specific. It had a higher capacity and was cheaper to perform than the immunoperoxidase monolayer assay and the indirect Elisa. It was comparable to the immunoperoxidase monolayer assay and better than the indirect Elisa in detecting antibodies formed early after infection, and it was superior to both the immunoperoxidase monolayer assay and the indirect Elisa in detecting antibodies at a late stage of infection.

KW - PRRS diagnosis virus

KW - antibodies

KW - porcine reproductive and respiratory syndrome virus

KW - ELISA

M3 - Journal article

SN - 0378-1135

VL - 56

SP - 1

EP - 8

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 1-2

ER -

Evaluation of a blocking ELISA for screening of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus

Abstract

Keywords

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