Evaluating robustness of a diesel-degrading bacterial consortium isolated from contaminated soil

Mateusz Sydow, Mikolaj Owsianiak, Zuzanna Szczepaniak, Grzegorz Framski, Barth F. Smets, Łukasz Ławniczak, Piotr Lisiecki, Alicja Szulc, Paweł Cyplik, Łukasz Chrzanowski

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Abstract

It is not known whether diesel-degrading bacterial communities are structurally and functionally robust when exposed to different hydrocarbon types. Here, we exposed a diesel-degrading consortium to model either alkanes, cycloalkanes or aromatic hydrocarbons as carbon sources to study its structural resistance. The structural resistance was low, with changes in relative abundances of up to four orders of magnitude, depending on hydrocarbon type and bacterial taxon. This low resistance is explained by the presence of hydrocarbon-degrading specialists in the consortium and differences in growth kinetics on individual hydrocarbons. However, despite this low resistance, structural and functional resilience were high, as verified by re-exposing the hydrocarbon-perturbed consortium to diesel fuel. The high resilience is either due to the short exposure time, insufficient for permanent changes in consortium structure and function, or the ability of some consortium members to be maintained during exposure on degradation intermediates produced by other members. Thus, the consortium is expected to cope with short-term exposures to narrow carbon feeds, while maintaining its structural and functional integrity, which remains an advantage over biodegradation approaches using single species cultures.
Original languageEnglish
JournalNew Biotechnology
Volume33
Issue number6
Pages (from-to)852-859
ISSN1871-6784
DOIs
Publication statusPublished - 2016

Keywords

  • Biotechnology
  • Molecular Biology
  • Bioengineering
  • Biodegradation
  • Community dynamics
  • Hydrocarbon
  • Resilience
  • Robustness
  • Diesel fuels
  • Growth kinetics
  • Hydrocarbons
  • Robustness (control systems)
  • Soil pollution
  • Bacterial community
  • Bacterial consortium
  • Degradation intermediates
  • Functional integrities
  • Orders of magnitude
  • Structural resistance
  • Aromatic hydrocarbons

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