Ethanol production from glucose and xylose by immobilized Thermoanaerobacter pentosaceus at 70 °C in an up-flow anaerobic sludge blanket (UASB) reactor

Sureewan Sittijunda, Ana Faria Tomás, Alissara Reungsang, Sompong O-thong, Irini Angelidaki

Research output: Contribution to journalJournal articleResearchpeer-review


The newly isolated extreme thermophilic ethanologen Thermoanaerobacter pentosaceus was immobilized in different support materials in order to improve its ethanol production ability. In batch fermentation, a maximum ethanol yield of 1.36 mol mol-1 consumed sugars was obtained by T. pentosaceus immobilized on rapeseed straw. Additionally, immobilized T. pentosaceus’ ethanol production was improved by 11 % in comparison to free cells. In continuous mode, it was shown that hydraulic retention time (HRT) affected ethanol yield, and a dramatic shift from ethanol to acetate and lactate production occurred at an HRT of 6 h. The maximum ethanol yield and concentration, 1.50 mol mol-1 consumed sugars and 12.4 g l-1, were obtained with an HRT of 12 h. The latter represented an improvement of 60 % in relation to previously obtained results. This indicates that immobilization of T. pentosaceus is an effective strategy to improve its ethanol production ability.
Original languageEnglish
JournalBioresource Technology
Pages (from-to)598–607
Publication statusPublished - 2013


  • Bioethanol
  • Extreme thermophilic
  • Immobilization
  • Thermoanaerobacter pentosaceus
  • Second-generation

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