TY - JOUR
T1 - Estimation of serum concentration of parvovirus B19 DNA by PCR in patients with chronic anemia
AU - Hornsleth, A.
AU - Carlsen, K. M.
AU - Christensen, Laurids Siig
AU - Gundestrup, M.
AU - Heegaard, E. D.
AU - Myhre, J.
PY - 1994
Y1 - 1994
N2 - Parvovirus B19 DNA was detected in serum samples from 10 out of 42 patients with chronic anaemia, the majority of whom suffered from aplastic anaemia, haemolytic anaemia, pure red cell anaemia or myelodysplastic syndrome. Nested PCR methods with sensitivities of 0.005-0.05 fg DNA were developed. In nine patients, B19 DNA could only be detected by nested PCR. Conventional PCR with a sensitivity of 50 fg B19 DNA could only detect B19 DNA in one patient. In the majority of B19-DNA-positive patients, the DNA concentration was estimated at 0.005-0.05 fg per 5 mu l serum.
AB - Parvovirus B19 DNA was detected in serum samples from 10 out of 42 patients with chronic anaemia, the majority of whom suffered from aplastic anaemia, haemolytic anaemia, pure red cell anaemia or myelodysplastic syndrome. Nested PCR methods with sensitivities of 0.005-0.05 fg DNA were developed. In nine patients, B19 DNA could only be detected by nested PCR. Conventional PCR with a sensitivity of 50 fg B19 DNA could only detect B19 DNA in one patient. In the majority of B19-DNA-positive patients, the DNA concentration was estimated at 0.005-0.05 fg per 5 mu l serum.
M3 - Journal article
SN - 0923-2516
VL - 145
SP - 379
EP - 386
JO - Research in Virology
JF - Research in Virology
IS - 6
ER -