Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine

Irina Borodina, Kanchana Rueksomtawin Kildegaard, Niels Bjerg Jensen, Thomas H. Blicher, Jerome Maury, Svetlana Sherstyk, Konstantin Schneider, Pedro Lamosa, Markus Herrgard, Inger Rosenstand, Fredrik Öberg, Jochen Förster, Jens Nielsen

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Microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global CO2 emissions. 3-Hydroxypropionic acid (3HP) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. With the objective of developing Saccharomyces cerevisiae as an efficient cell factory for highlevel production of 3HP, we identified the ß-alanine biosynthetic route as the most economically attractive according to the metabolic modeling. We engineered and optimized a synthetic pathway for de novo biosynthesis of ß-alanine and its subsequent conversion into 3HP using a novel ß-alanine-pyruvate aminotransferase discovered in Bacillus cereus. The final strain produced 3HP at a titer of 13.7±0.3 g・L-1 with a 0.14±0.0 C-mol・C-mol-1 yield on glucose in 80 hours in controlled fed-batch fermentation in mineral medium at pH 5, and this work therefore lays the basis for developing a process for biological 3HP production.
Original languageEnglish
JournalMetabolic Engineering
Pages (from-to)57-64
Publication statusPublished - 2015

Bibliographical note

This is an open access article under the CC BY license (


  • Biosustainable acrylics
  • 3-hydroxypropionic acid
  • Saccharomyces cerevisiae
  • β-alanine
  • β-alanine-pyruvate aminotransferase

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