ERM immersion vaccination and adjuvants

J. Skov, J. K. Chettri, R. M. Jaafar, P. W. Kania, Inger Dalsgaard, K. Buchmann

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    Two candidate adjuvants were tested with a commercial ERM dip vaccine (AquaVac™ Relera, MSD Animal Health) for rainbow trout in an experimental design compatible with common vaccination practices at farm level, i.e. immersion of fish in vaccine (±adjuvant) for 30 s. The adjuvants were the commercial product Montanide™ IMS 1312 VG PR (SEPPIC), and a soluble and ≥98% pure β-glucan from yeast (Saccharomyces cerevisiae) (Sigma-Aldrich). Hence, five experimental groups in duplicate were established and exposed to vaccine and adjuvants in the following combinations: AquaVac™ Relera (alone); AquaVac™ Relera + Montanide™; AquaVac™ Relera + β-glucan; Montanide™ (alone); and β-glucan (alone). Approximately 450 degree days post-vaccination, the fish were bath-challenged with live Yersinia ruckeri to produce survival curves. Blood, skin and gills were sampled at selected time points during the course of the experiment to test for plasma Ab levels and lysozyme activity, and the regulation of immune relevant genes and cells in external, mucosal tissues. Preliminary results show 96% to 100% survival of vaccinated fish with and without any of the two adjuvants, whereas unvaccinated controls and fish exposed to β-glucan alone experienced 58% and 60% survival, respectively (calculated at day 24 post-challenge). Montanide™ alone gave rise to an intermediate level of 72% survival. Lysozyme activity levels in plasma were markedly elevated at day 3 and day 24 post-challenge in fish exposed to Montanide™ alone or β-glucan alone compared to fish from any of the three vaccinated groups.
    Original languageEnglish
    Title of host publicationDafinet and Profish : Book of abstracts
    Number of pages1
    Place of PublicationFrederiksberg C
    PublisherUniversity of Copenhagen
    Publication date2015
    Publication statusPublished - 2015
    EventDAFINET and ProFish Workshop - København, Denmark
    Duration: 17 Nov 201518 Nov 2015


    ConferenceDAFINET and ProFish Workshop

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