Epidemiological cut-off values for Vibrio anguillarum MIC and disc diffusion data generated by standardised methods

Peter Smith*, Laëtitia Le Devendec, Eric Jouy, Emeline Larvor, Alain Le Breton, Sara Picon-Camacho, Snježana Zrnčić, Ivana Giovanna Zupičić, Dražen Oraić, Süheyla Karataş, David Verner-Jeffreys, Andrew Wokorac Joseph, Edel Light, Alieda van Essen-Zandbergen, Betty van Gelderen, Michal Voorbergen-Laarman, Olga L.M. Haenen, Kees T. Veldman, Lone Madsen, Kári K. MouritsenCecilie Smith Svanevik, Fredrik Håkonsholm, Ana Isabel Vela, María García, Daniela Florio, Marialetizia Fioravanti, Luana Cortinovis, Tobia Pretto, Amedeo Manfrin, Sandrine Baron

*Corresponding author for this work

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This work aims to generate the data needed to set epidemiological cut-off values for minimum inhibitory concentration (MIC) and disc-diffusion zone measurements of Vibrio anguillarum. A total of 261 unique isolates were tested, applying standard methods specifying incubation at 28°C for 24−28 h. Aggregated MIC distributions for a total of 247 isolates were determined in 9 laboratories for 11 agents. Data aggregations of the disc zone for the 10 agents analysed contained between 157 and 218 observations made by 4 to 7 laboratories. Acceptable ranges for quality control (QC) reference strains were available for 7 agents and the related multi-laboratory aggregated data were censored, excluding the data of a laboratory that failed to meet QC requirements. Statistical methods were applied to calculate epidemiological cut-off values. Cut-off values for MIC data were calculated for florfenicol (≤1 μg ml−1), gentamicin (≤4 μg ml−1), oxytetracycline (≤0.25 μg ml−1) and trimethoprim/sulfamethoxazole (≤0.125/2.38 μg ml−1). The cut-off values for disc zone data were calculated for enrofloxacin (≥29 mm), florfenicol (≥27 mm), gentamicin (≥19 mm), oxolinic acid (≥24 mm), oxytetracycline (≥24 mm) and trimethoprim/sulfamethoxazole (≥26 mm). MIC and disc-diffusion zone data for the other agents where not supported by QC, thus yielding only provisional cutoff values (meropenem, ceftazidime). Regardless of whether QC is available, some of the aggregated MIC distributions (enrofloxacin, oxolinic acid), disc zone (sulfamethoxazole), and MIC and disc-diffusion distributions (ampicillin, chloramphenicol) did not meet the statistical requirements. The data produced will be submitted to the Clinical Laboratory Standards Institute for their consideration in setting international consensus epidemiological cut-off values.
Original languageEnglish
JournalDiseases of Aquatic Organisms
Pages (from-to)109-123
Number of pages15
Publication statusPublished - 2023


  • CLSI VET04
  • Normalized resistance interpretation
  • ECOFFinder
  • Antimicrobial suceptibility
  • Aquatic animals


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