TY - JOUR
T1 - Epidemiological cut-off values for Vibrio anguillarum MIC and disc diffusion data generated by standardised methods
AU - Smith, Peter
AU - Le Devendec, Laëtitia
AU - Jouy, Eric
AU - Larvor, Emeline
AU - Le Breton, Alain
AU - Picon-Camacho, Sara
AU - Zrnčić, Snježana
AU - Zupičić, Ivana Giovanna
AU - Oraić, Dražen
AU - Karataş, Süheyla
AU - Verner-Jeffreys, David
AU - Joseph, Andrew Wokorac
AU - Light, Edel
AU - van Essen-Zandbergen, Alieda
AU - van Gelderen, Betty
AU - Voorbergen-Laarman, Michal
AU - Haenen, Olga L.M.
AU - Veldman, Kees T.
AU - Madsen, Lone
AU - Mouritsen, Kári K.
AU - Svanevik, Cecilie Smith
AU - Håkonsholm, Fredrik
AU - Vela, Ana Isabel
AU - García, María
AU - Florio, Daniela
AU - Fioravanti, Marialetizia
AU - Cortinovis, Luana
AU - Pretto, Tobia
AU - Manfrin, Amedeo
AU - Baron, Sandrine
PY - 2023
Y1 - 2023
N2 - This work aims to generate the data needed to set epidemiological cut-off values for minimum inhibitory concentration (MIC) and disc-diffusion zone measurements of Vibrio anguillarum. A total of 261 unique isolates were tested, applying standard methods specifying incubation at 28°C for 24−28 h. Aggregated MIC distributions for a total of 247 isolates were determined in 9 laboratories for 11 agents. Data aggregations of the disc zone for the 10 agents analysed contained between 157 and 218 observations made by 4 to 7 laboratories. Acceptable ranges for quality control (QC) reference strains were available for 7 agents and the related multi-laboratory aggregated data were censored, excluding the data of a laboratory that failed to meet QC requirements. Statistical methods were applied to calculate epidemiological cut-off values. Cut-off values for MIC data were calculated for florfenicol (≤1 μg ml−1), gentamicin (≤4 μg ml−1), oxytetracycline (≤0.25 μg ml−1) and trimethoprim/sulfamethoxazole (≤0.125/2.38 μg ml−1). The cut-off values for disc zone data were calculated for enrofloxacin (≥29 mm), florfenicol (≥27 mm), gentamicin (≥19 mm), oxolinic acid (≥24 mm), oxytetracycline (≥24 mm) and trimethoprim/sulfamethoxazole (≥26 mm). MIC and disc-diffusion zone data for the other agents where not supported by QC, thus yielding only provisional cutoff values (meropenem, ceftazidime). Regardless of whether QC is available, some of the aggregated MIC distributions (enrofloxacin, oxolinic acid), disc zone (sulfamethoxazole), and MIC and disc-diffusion distributions (ampicillin, chloramphenicol) did not meet the statistical requirements. The data produced will be submitted to the Clinical Laboratory Standards Institute for their consideration in setting international consensus epidemiological cut-off values.
AB - This work aims to generate the data needed to set epidemiological cut-off values for minimum inhibitory concentration (MIC) and disc-diffusion zone measurements of Vibrio anguillarum. A total of 261 unique isolates were tested, applying standard methods specifying incubation at 28°C for 24−28 h. Aggregated MIC distributions for a total of 247 isolates were determined in 9 laboratories for 11 agents. Data aggregations of the disc zone for the 10 agents analysed contained between 157 and 218 observations made by 4 to 7 laboratories. Acceptable ranges for quality control (QC) reference strains were available for 7 agents and the related multi-laboratory aggregated data were censored, excluding the data of a laboratory that failed to meet QC requirements. Statistical methods were applied to calculate epidemiological cut-off values. Cut-off values for MIC data were calculated for florfenicol (≤1 μg ml−1), gentamicin (≤4 μg ml−1), oxytetracycline (≤0.25 μg ml−1) and trimethoprim/sulfamethoxazole (≤0.125/2.38 μg ml−1). The cut-off values for disc zone data were calculated for enrofloxacin (≥29 mm), florfenicol (≥27 mm), gentamicin (≥19 mm), oxolinic acid (≥24 mm), oxytetracycline (≥24 mm) and trimethoprim/sulfamethoxazole (≥26 mm). MIC and disc-diffusion zone data for the other agents where not supported by QC, thus yielding only provisional cutoff values (meropenem, ceftazidime). Regardless of whether QC is available, some of the aggregated MIC distributions (enrofloxacin, oxolinic acid), disc zone (sulfamethoxazole), and MIC and disc-diffusion distributions (ampicillin, chloramphenicol) did not meet the statistical requirements. The data produced will be submitted to the Clinical Laboratory Standards Institute for their consideration in setting international consensus epidemiological cut-off values.
KW - CLSI VET04
KW - Normalized resistance interpretation
KW - ECOFFinder
KW - Antimicrobial suceptibility
KW - Aquatic animals
U2 - 10.3354/dao03745
DO - 10.3354/dao03745
M3 - Journal article
C2 - 37650482
SN - 0177-5103
VL - 155
SP - 109
EP - 123
JO - Diseases of Aquatic Organisms
JF - Diseases of Aquatic Organisms
ER -