Epi-detecting label-free multimodal imaging platform using a compact diode-pumped femtosecond solid-state laser

Marco Andreana, Tuan Le, Anders Kragh Hansen, Aart J. Verhoef, Ole Bjarlin Jensen, Peter E. Andersen, Paul Slezak, Wolfgang Drexler, Alma Fernández, Angelika Unterhuber

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We have developed an epi-detected multimodal nonlinear optical microscopy platform based on a compact and cost-effective laser source featuring simultaneous acquisition of signals arising from hyperspectral coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence, and second harmonic generation. The laser source is based on an approach using a frequency-doubled distributed Bragg reflector-tapered diode laser to pump a femtosecond Ti:sapphire laser. The operational parameters of the laser source are set to the optimum trade-off between the spectral and temporal requirements for these three modalities, achieving sufficient spectral resolution for CARS in the lipid region. The experimental results on a biological tissue reveal that the combination of the epi-detection scheme and the use of a compact diode-pumped femtosecond solid-state laser in the nonlinear optical microscope is promising for biomedical applications in a clinical environment
Original languageEnglish
Article number091517
JournalJournal of Biomedical Optics
Issue number9
Number of pages8
Publication statusPublished - 2017

Bibliographical note

© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.


  • Diode lasers
  • Solid-state lasers
  • Nonlinear optics
  • Scanning microscopy
  • Raman spectroscopy
  • Hyperspectral imaging


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