ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR SCREENING OF MILK SAMPLES FOR SALMONELLA-TYPHIMURIUM IN DAIRY HERDS

Jeffrey Hoorfar, A. Wedderkopp

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We investigated the ability of an antibody-specific, O antigen-based ELISA to document Salmonella typhimurium herd infections by screening of milk samples. Three cattle populations, 20 herds with no history of salmonellosis, 8 herds with history of S typhimurium epsiodes within the previous 7 months, and 220 herds of unknown disease status, were tested. A herd was considered ELISA positive if at least 5% of the cows had OD values > 0.3. Among the 20 herds without history of salmonellosis, only 2 herds were ELISA positive, whereas all 8 herds with a known history of salmonellosis were ELISA positive (herd specificity, 0.9 and herd sensitivity, 1.0). A sig nificant correlation (P <0.001) was found between the OD values of serum and milk samples from cows in the herds with a history of salmonellosis. It was concluded that ELISA testing of individual milk sam ples can be used for surveillance of herds for S typhimurium infections, but further modifications are needed to test bulk tank milk samples.
Original languageEnglish
JournalAmerican Journal of Veterinary Research
Volume56
Issue number12
Pages (from-to)1549-1554
ISSN0002-9645
Publication statusPublished - 1995

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