Enzyme Enhanced Protein Recovery from Green Biomass Pulp

Gleb Dotsenko, Lene Lange

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Globally, animal feed protein is a key factor for production of meat for human consumption. Protein for animal feed is in many parts of the world not available in sufficient amounts; demand is met only through import of feed protein. Such protein deficit can be minimized through optimized use of local protein resources based on upgrade from e.g. green plant biomass. In present work we consider different strategies for protein recovery from white clover and ryegrass screw press pulps, using aqueous extraction, as well as carbohydrases and proteases enhanced extraction. Protein recovery in these studies was determined as a yield of solubilized protein with regard to the total protein in a screw press pulp. Aqueous extraction at pH 8.0 resulted in approx. 40 % protein recovery, while proteases application (Savinase 16.0L, Novozymes) enabled twice higher protein yield. Application of plant cell wall degrading enzymes (Cellic CTec2 and Cellic HTec2, Novozymes) did not provide detectable protein recovery, while consecutive proteases treatment resulted in approx. 95 % protein yield. RuBisCO peptides were demonstrated by amino acid analysis to be the major component of white clover and ryegrass pulp proteolyzates, generated by Savinase 16.0L protease.
Original languageEnglish
JournalWaste and Biomass Valorization
Issue number4
Pages (from-to)1257–1264
Publication statusPublished - 2017

Bibliographical note

Copyright:The Author(s) 2016. This article is published with open access at Springerlink.com


  • Carbohydrases
  • Leaf protein
  • Proteases
  • Ryegrass
  • Screw press pulp
  • White clover


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