TY - JOUR
T1 - Enzymatic polymerization of polythiophene by immobilized glucose oxidase
AU - Krikstolaityte, Vida
AU - Kuliesius, Jurgis
AU - Ramanaviciene, Almira
AU - Mikoliunaite, Lina
AU - Kausaite-Minkstimiene, Asta
AU - Oztekin, Yasemin
AU - Ramanavicius, Arunas
PY - 2014
Y1 - 2014
N2 - In this study 'green', environmentally friendly enzymatic reaction-based synthesis of conducting polymer polythiophene (PTP) is proposed. Glucose oxidase (GOx) was shown as an effective catalyst, which, in the presence of glucose, produces hydrogen peroxide suitable for the oxidative polymerization of PTP under ambient conditions at neutral pH. Enzymatically induced formation of the PTP layer over GOx-modified graphite rod electrode (GRE) was demonstrated and evaluated amperometrically and by attenuated total reflectance - Fourier transform infrared (ATR-FTIR) spectroscopy. Surface morphology of GOx- and PTP-modified GR electrodes was characterized by atomic force microscopy. It was clearly shown that the apparent kinetic Michaelis constant (KM(app.)) of GOx/PTP-modified GRE increased by increasing the duration of polymerization reaction. Therefore, enzymatic polymerization could be applied in adjustment and/or tuning of KM(app.) and other kinetic parameters of GOx-based electrodes used in biosensor design. (C) 2014 Elsevier Ltd. All rights reserved.
AB - In this study 'green', environmentally friendly enzymatic reaction-based synthesis of conducting polymer polythiophene (PTP) is proposed. Glucose oxidase (GOx) was shown as an effective catalyst, which, in the presence of glucose, produces hydrogen peroxide suitable for the oxidative polymerization of PTP under ambient conditions at neutral pH. Enzymatically induced formation of the PTP layer over GOx-modified graphite rod electrode (GRE) was demonstrated and evaluated amperometrically and by attenuated total reflectance - Fourier transform infrared (ATR-FTIR) spectroscopy. Surface morphology of GOx- and PTP-modified GR electrodes was characterized by atomic force microscopy. It was clearly shown that the apparent kinetic Michaelis constant (KM(app.)) of GOx/PTP-modified GRE increased by increasing the duration of polymerization reaction. Therefore, enzymatic polymerization could be applied in adjustment and/or tuning of KM(app.) and other kinetic parameters of GOx-based electrodes used in biosensor design. (C) 2014 Elsevier Ltd. All rights reserved.
U2 - 10.1016/j.polymer.2014.02.003
DO - 10.1016/j.polymer.2014.02.003
M3 - Journal article
VL - 55
SP - 1613
EP - 1620
JO - Polymer
JF - Polymer
SN - 0032-3861
IS - 7
ER -