Enzymatic network for production of ether amines from alcohols

Cyntia M. Palacio, Ciprian G. Crismaru, Sebastian Bartsch, Vaidotas Navickas, Klaus Ditrich, Michael Breuer, Rohana Abu, John Woodley, Kai Baldenius, Bian Wu, Dick B. Janssen

Research output: Contribution to journalJournal article

Abstract

We constructed an enzymatic network composed of three different enzymes for the synthesis of valuable ether amines. The enzymatic reactions are interconnected to catalyze the oxidation and subsequent transamination of the substrate and to provide cofactor recycling. This allows production of the desired ether amines from the corresponding ether alcohols with inorganic ammonium as the only additional substrate. To examine conversion, individual and overall reaction equilibria were established. Using these data, it was found that the experimentally observed conversions of up to 60% observed for reactions containing 10mM alcohol and up to 280mM ammonia corresponded well to predicted conversions. The results indicate that efficient amination can be driven by high concentrations of ammonia and may require improving enzyme robustness for scale-up.
Original languageEnglish
JournalBiotechnology and Bioengineering
Volume113
Issue number9
Pages (from-to)1853–1861
ISSN0006-3592
DOIs
Publication statusPublished - 2016

Keywords

  • Alcohol dehydrogenase
  • Amination
  • Aminotransferase
  • Biocatalysis
  • Ether amines

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