Enzymatic Modification of Sphingomyelin

Long Zhang (Author), Lars Hellgren (Author), Xuebing Xu (Author)

    Research output: Non-textual formSound/Visual production (digital)Research


    Due to its major role in maintaining the water-retaining properties of the epidermis, ceramide is of great commercial potential in cosmetic and pharmaceuticals such as hair and skin care products. Currently, chemical synthesis of ceramide is a costly process, and developments of alternative cost-efficient, high yield production methods are of great interest. In the present study, the potential of producing ceramide through the enzymatic hydrolysis of sphingomyelin have been studied. sphingomyelin is a ubiquitous membrane-lipid and rich in dairy products or by-products. It has been verified that sphingomyelin modification gives a feasible approach to the potential production of ceramide. The reaction system has been improved through system evaluation and the optimization of several important factors, and phospholipase C from Clostridium perfringens shows higher activity towards the hydrolysis reaction. From several particles which the enzyme was immobilized on, Lewatit VP OC 1600 (Bayer AG) is the most desirable as a consequence of the highest retention activity of the immobilized enzyme. The further research focus on the immobilization using this carrier was conducted. This presentation will describe the research background, introduce the basic reaction system with its optimisation, and release the recent results in the immobilization study.
    Original languageEnglish
    Publication date2006
    Publication statusPublished - 2006
    Event97th AOCS Annual Meeting & Expo - St. Louis, MO, United States
    Duration: 30 Apr 20063 May 2006
    Conference number: 97


    Conference97th AOCS Annual Meeting & Expo
    Country/TerritoryUnited States
    CitySt. Louis, MO
    Internet address


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