TY - JOUR
T1 - Enzymatic extraction of antioxidant ingredients from Danish seaweeds and characterization of active principles
AU - Habeebullah, Sabeena Farvin Koduvayur
AU - Alagarsamy, Surendraraj
AU - Arnous, Anis
AU - Jacobsen, Charlotte
PY - 2021
Y1 - 2021
N2 - The present study investigated the enzymatic extraction of antioxidant ingredients from four Danish seaweeds viz., Fucus vesiculosus, Fucus serratus, Ascophyllum nodosum and Polysiphonia fucoides. The seaweeds were hydrolyzed with five carbohydrases and three proteases. The antioxidant activities and total phenolic content (TPC) of the resulting extracts were studied. Considerable variation was observed in the antioxidant activities and TPC of different enzymatic hydrolysates. Among the 32 enzymatic extracts studied, F. vesiculosus-Alcalase, F. vesiculosus-Viscozyme, F. serratus-Termamyl, and P. fucoides-Termamyl extracts showed high TPC and antioxidant activities. Fractionation and characterization of these extracts revealed that radical scavenging and reducing power was mainly due to the phenolic fractions, whereas protein and polysaccharide fractions mainly contributed to the iron-chelating activity. The antioxidant activity observed in the liposome model system was due to a combined effect of the various fractions and their antioxidant properties. Gallic, gentisic and chlorogenic acids were the main free phenolics in the phenolic fractions. Fucose rich polysaccharides were obtained when F. vesiculosus and F. serratus were hydrolyzed with Viscozyme and Termamyl, respectively. Thus, enzyme assisted extraction is an effective tool for getting antioxidant with specific properties, which can find application in food and cosmetics.
AB - The present study investigated the enzymatic extraction of antioxidant ingredients from four Danish seaweeds viz., Fucus vesiculosus, Fucus serratus, Ascophyllum nodosum and Polysiphonia fucoides. The seaweeds were hydrolyzed with five carbohydrases and three proteases. The antioxidant activities and total phenolic content (TPC) of the resulting extracts were studied. Considerable variation was observed in the antioxidant activities and TPC of different enzymatic hydrolysates. Among the 32 enzymatic extracts studied, F. vesiculosus-Alcalase, F. vesiculosus-Viscozyme, F. serratus-Termamyl, and P. fucoides-Termamyl extracts showed high TPC and antioxidant activities. Fractionation and characterization of these extracts revealed that radical scavenging and reducing power was mainly due to the phenolic fractions, whereas protein and polysaccharide fractions mainly contributed to the iron-chelating activity. The antioxidant activity observed in the liposome model system was due to a combined effect of the various fractions and their antioxidant properties. Gallic, gentisic and chlorogenic acids were the main free phenolics in the phenolic fractions. Fucose rich polysaccharides were obtained when F. vesiculosus and F. serratus were hydrolyzed with Viscozyme and Termamyl, respectively. Thus, enzyme assisted extraction is an effective tool for getting antioxidant with specific properties, which can find application in food and cosmetics.
KW - Antioxidants
KW - Phenolics
KW - Polysaccharides
KW - Proteins
KW - Seaweed
U2 - 10.1016/j.algal.2021.102292
DO - 10.1016/j.algal.2021.102292
M3 - Journal article
SN - 2211-9264
VL - 56
JO - Algal Research
JF - Algal Research
M1 - 102292
ER -