Enzymatic extraction improves intracellular protein recovery from the industrial carrageenan seaweed Eucheuma denticulatum revealed by quantitative, subcellular protein profiling: A high potential source of functional food ingredients

Simon Gregersen*, Anne-Sofie Havgaard Kongsted, Rikke Brønnum Nielsen, Søren Storck Hansen, Frederik Andersen Lau, Jacob Bisgaard Rasmussen, Susan Løvstad Holdt, Charlotte Jacobsen

*Corresponding author for this work

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Abstract

Seaweeds are regarded as a sustainable source of food protein, but protein extraction is severely impaired by the complex extracellular matrix. In this work, we investigated the protein-level effects of enzymatic extraction upstream of carrageenan extraction for the industrial red seaweed Eucheuma denticulatum. Combination of quantitative proteomics and bioinformatic prediction of subcellular localization was shown to have immense potential for process evaluation; even in the case of poorly annotated species such as E. denticulatum. Applying cell wall degrading enzymes markedly improved the relative recovery of intracellular proteins compared to treatment with proteolytic enzymes or no enzymatic treatment. Moreover, results suggest that proteomics data may prove useful for characterizing amino acid composition and that length-normalization is a viable approach for relative protein quantification in non-specific analysis. Importantly, the extracts were abundant in proteins, which contained both previously verified and novel, potential bioactive peptides, highlighting their potential for application as functional food ingredients.
Original languageEnglish
Article number100137
JournalFood Chemistry: X
Volume12
Number of pages13
ISSN2590-1575
DOIs
Publication statusPublished - 2021

Keywords

  • Macroalgae
  • Enzymatic protein extraction
  • Proteomics
  • Bioinformatics
  • Process evaluation
  • Bioactive peptides

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