Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery

Jesper Davidsen, C. Vermehren, S. Frøkjær, Ole G. Mouritsen, Kent Jørgensen

Research output: Contribution to journalJournal articleResearchpeer-review


Polyethylenoxide (PEG) covered liposomes are used as lipid-based drug-delivery systems. In comparison to conventional liposomes the polymer-covered liposomes display a long circulation half-life in the blood stream. We investigate the influence of polyethyleneoxide-distearoylphosphatidylethanolamine (DSPE-PEG$-750$/) lipopolymer concentration on phospholipase A$-2$/ (PLA$-2$/) catalyzed hydrolysis of liposomes composed of stearoyloleoylphosphatidylcholine (SOPC). The characteristic PLA$-2$/ lag-time was determined by fluorescence and the degree of lipid hydrolysis was followed by HPLC analysis. Particle size and zeta-potential were measured as a function of DSPE-PEG$-750$/ lipopolymer concentration. A significant decrease in the lag-time, and hence an increase in enzyme activity, was observed with increasing concentrations of the anionic DSPE-PEG$-750$/ lipopolymer lipids. The observed decrease in lag-time might be related to changes in the surface potential and the PLA$-2$/ lipid membrane affinity.
Original languageEnglish
JournalAdvances in Colloid and Interface Science
Pages (from-to)303-311
Publication statusPublished - 2001

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