Environmental levels of the antidepressant venlafaxine impact the metabolic capacity of rainbow trout

Carol Best, Nataliya Melnyk-Lamont, Manuel Gesto, Mathilakath M. Vijayan

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The antidepressant venlafaxine is detected at parts per billion levels in tertiary-treated municipal wastewater effluent. However, the impact of this serotonin-norepinephrine reuptake inhibitor (SNRI) on non-target aquatic animals is poorly understood. We tested the hypothesis that environmentally relevant levels of venlafaxine disrupt the highly conserved cortisol and glucose response to stress in rainbow trout (Oncorhynchus mykiss). Juvenile trout were exposed to venlafaxine (0, 0.2 and 1.0 mu g/L) in a static system with daily renewal for seven days. The fish were then subjected to an acute handling disturbance and sampled either prior to (0 h) or 1,4 and 24h after stressor exposure. Venlafaxine exposure did not affect the handling disturbance-mediated transient elevation in plasma cortisol levels or target tissue glucocorticoid receptor expression. The drug exposure disrupted the interrenal steroidogenic capacity, including altered handling stressor-mediated changes in mRNA abundances of steroidogenic acute regulatory protein and cytochrome P450 side chain cleavage. The handling stressor-induced transient elevations in plasma glucose levels were significantly reduced in the venlafaxine-exposed fish. This was not accompanied by changes in liver glycogen content, glucose transporter 2 mRNA abundance or the glycolytic capacity, whereas the capacity for gluconeogenesis and amino acid catabolism were enhanced. Venlafaxine also brought about changes in the gill of trout, including enhanced lactate dehydrogenase activity and Na+-K+ ATPase protein expression, while the Na+-K+ ATPase enzyme activity was reduced. Collectively, our results demonstrate that venlafaxine at levels detected in the aquatic environment impacts tissue metabolic capacities and may compromise the adaptive responses to an acute stressor in rainbow trout. (C) 2014 Elsevier B.V. All rights reserved.
Original languageEnglish
JournalAquatic Toxicology
Volume155
Issue number7
Pages (from-to)190-198
ISSN0166-445X
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • MARINE
  • TOXICOLOGY
  • ACUTE REGULATORY PROTEIN
  • SIDE-CHAIN CLEAVAGE
  • ONCORHYNCHUS-MYKISS
  • STRESS-RESPONSE
  • STAR PROTEIN
  • FISH
  • CORTISOL
  • LIVER
  • PHARMACEUTICALS
  • WATER
  • Fish
  • Salmonid
  • Environmental pharmaceuticals
  • Serotonin-norepinephrine reuptake inhibitor
  • Stress response
  • Intermediary metabolism
  • Aquatic Science
  • Health, Toxicology and Mutagenesis
  • adenosine triphosphatase (potassium sodium)
  • cytochrome P450
  • glucocorticoid receptor
  • glucose
  • glucose transporter 2
  • hydrocortisone
  • lactate dehydrogenase
  • venlafaxine
  • aquatic environment
  • drug
  • effluent
  • environmental impact
  • enzyme activity
  • metabolism
  • pollution exposure
  • salmonid
  • wastewater
  • amino acid metabolism
  • amino acid sequence
  • animal experiment
  • animal tissue
  • article
  • controlled study
  • down regulation
  • environmental exposure
  • environmental stress
  • gluconeogenesis
  • glucose transporter 2 gene
  • glycogen analysis
  • glycolysis
  • hydrocortisone blood level
  • metabolic capacity
  • nonhuman
  • nucleotide sequence
  • priority journal
  • protein expression
  • rainbow trout
  • steroidogenesis
  • Animals
  • Antidepressive Agents
  • Blood Glucose
  • Cyclohexanols
  • Gluconeogenesis
  • Glucose
  • Glycolysis
  • Handling (Psychology)
  • Hydrocortisone
  • Liver
  • Liver Glycogen
  • Oncorhynchus mykiss
  • Receptors, Glucocorticoid
  • Stress, Physiological
  • Water Pollutants, Chemical
  • Serotonin–norepinephrine reuptake inhibitor
  • Pisces Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Osteichthyes [85206] Oncorhynchus mykiss species rainbow trout common immature
  • cortisol 50-23-7
  • cytochrome P450 9035-51-2 EC 1.14.14.1
  • glucocorticoid receptor expression
  • glycogen 9005-79-2
  • lactate dehydrogenase 9001-60-9 EC 1.1.1.27
  • messenger RNA
  • serotonin-norepinephrine reuptake inhibitor SNRI
  • sodium ion-potassium ion ATPase expression EC 3.6.3.9
  • venlafaxine 93413-69-5 antidepressant-drug toxin
  • 10060, Biochemistry studies - General
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10064, Biochemistry studies - Proteins, peptides and amino acids
  • 10067, Biochemistry studies - Sterols and steroids
  • 10068, Biochemistry studies - Carbohydrates
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 12512, Pathology - Therapy
  • 14004, Digestive system - Physiology and biochemistry
  • 15002, Blood - Blood and lymph studies
  • 15004, Blood - Blood cell studies
  • 16004, Respiratory system - Physiology and biochemistry
  • 22026, Pharmacology - Psychopharmacology
  • 22501, Toxicology - General and methods
  • Biochemistry and Molecular Biophysics
  • gill respiratory system
  • liver digestive system
  • plasma blood and lymphatics
  • municipal wastewater applied and field techniques
  • Enzymology
  • Toxicology

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