Environmental factors affecting challenge success in vaccination studies

J. K. Chettri; J. Skov; Inger Dalsgaard; K. Buchman

Environmental factors affecting challenge success in vaccination studies

J. K. Chettri
, J. Skov
, Inger Dalsgaard
, K. Buchman

University of Copenhagen

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Research › peer-review

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Abstract

We have tested the efficacies of two different vaccines (a commercial versus an experimental vaccine – both being oil adjuvanted) for rainbow trout against furunculosis caused by Aeromonas salmonicida infections. However, when challenging fish with live bacteria in order to assess protection following vaccination, the administration of the pathogen is important for the outcome of the experiments. We have therefore also compared injection challenge with cohabitation challenge. In addition, when doing so we also investigated the influence of environmental conditions such as salinity and temperature on the protection recorded. Thus, challenge studies were conducted at two temperatures (12 and 19°C) and at two salinities (0 and 15 ppt). Mortalities following challenge were recorded and RPS calculated for each group. Side effects of the vaccines were evaluated by using the Speilberg scale. Results from the challenge studies will be presented.

Original language	English
Title of host publication	DAFINET Workshop and PhD Course : Book of Abstracts
Number of pages	1
Publication date	2013
Publication status	Published - 2013
Event	DAFINET Workshop : Fish Immunology: From Egg to Adult Fish - Frederiksberg, Denmark Duration: 12 Nov 2013 → 14 Nov 2013 http://www.dafinet.dk

Conference

Conference	DAFINET Workshop : Fish Immunology: From Egg to Adult Fish
Country/Territory	Denmark
City	Frederiksberg
Period	12/11/2013 → 14/11/2013
Internet address	http://www.dafinet.dk

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SDG 3 Good Health and Well-being

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title = "Environmental factors affecting challenge success in vaccination studies",

abstract = "We have tested the efficacies of two different vaccines (a commercial versus an experimental vaccine – both being oil adjuvanted) for rainbow trout against furunculosis caused by Aeromonas salmonicida infections. However, when challenging fish with live bacteria in order to assess protection following vaccination, the administration of the pathogen is important for the outcome of the experiments. We have therefore also compared injection challenge with cohabitation challenge. In addition, when doing so we also investigated the influence of environmental conditions such as salinity and temperature on the protection recorded. Thus, challenge studies were conducted at two temperatures (12 and 19°C) and at two salinities (0 and 15 ppt). Mortalities following challenge were recorded and RPS calculated for each group. Side effects of the vaccines were evaluated by using the Speilberg scale. Results from the challenge studies will be presented.",

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T1 - Environmental factors affecting challenge success in vaccination studies

AU - Chettri, J. K.

AU - Skov, J.

AU - Dalsgaard, Inger

AU - Buchman, K.

PY - 2013

Y1 - 2013

N2 - We have tested the efficacies of two different vaccines (a commercial versus an experimental vaccine – both being oil adjuvanted) for rainbow trout against furunculosis caused by Aeromonas salmonicida infections. However, when challenging fish with live bacteria in order to assess protection following vaccination, the administration of the pathogen is important for the outcome of the experiments. We have therefore also compared injection challenge with cohabitation challenge. In addition, when doing so we also investigated the influence of environmental conditions such as salinity and temperature on the protection recorded. Thus, challenge studies were conducted at two temperatures (12 and 19°C) and at two salinities (0 and 15 ppt). Mortalities following challenge were recorded and RPS calculated for each group. Side effects of the vaccines were evaluated by using the Speilberg scale. Results from the challenge studies will be presented.

AB - We have tested the efficacies of two different vaccines (a commercial versus an experimental vaccine – both being oil adjuvanted) for rainbow trout against furunculosis caused by Aeromonas salmonicida infections. However, when challenging fish with live bacteria in order to assess protection following vaccination, the administration of the pathogen is important for the outcome of the experiments. We have therefore also compared injection challenge with cohabitation challenge. In addition, when doing so we also investigated the influence of environmental conditions such as salinity and temperature on the protection recorded. Thus, challenge studies were conducted at two temperatures (12 and 19°C) and at two salinities (0 and 15 ppt). Mortalities following challenge were recorded and RPS calculated for each group. Side effects of the vaccines were evaluated by using the Speilberg scale. Results from the challenge studies will be presented.

M3 - Conference abstract in proceedings

BT - DAFINET Workshop and PhD Course

T2 - DAFINET Workshop : Fish Immunology: From Egg to Adult Fish

Y2 - 12 November 2013 through 14 November 2013

ER -

Environmental factors affecting challenge success in vaccination studies

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