Enkephalins: Raman spectral analysis and comparison as function of pH 1-13

Salim Abdali, Pia Refstrup, O.F. Nielsen, Henrik Bohr

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Raman spectral studies are carried out on Leu- and Met-enkephalin as a function of the pH value in the range of 1-13. The molecules are dissolved in KCI solvent and the pH is controlled at each value. Spectral analyses reveal the dependence of the structural conformation on the pH, and a comparison of the two molecules is made in three frequency regions: the tyrosine Fermi doublet (850-830 cm(-1)), aromatic side chains (1650-1550 cm(-1)), and carboxylate (1430-1400 cm(-1)). All regions and frequencies are presented, discussed, and compared for the two molecules.
Original languageEnglish
JournalBiopolymers
Volume72
Issue number5
Pages (from-to)318-328
ISSN0006-3525
Publication statusPublished - 2003

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