Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser

Gianni Nteroli*, Giulia Messa, Stella Koutsikou, Manoj K. Dasa, Antti Penttinen, Antti Härkönen, Mircea Guina, Adrian Podoleanu, Adrian Bradu

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

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Abstract

In conventional optoacoustic microscopy, nanosecond pulse duration lasers are employed. When a laser delivering shorter pulse durations is used, it is expected that, from a theoretical point of view, broader, higher-frequency acoustic waves to be generated, therefore a better axial resolution of the instrument. In the present report, this advantage, offered by a picosecond duration pulse laser, to experimentally demonstrate that the axial resolution of an optoacoustic microscopy instrument can be enhanced was exploited. In comparison to a 2 ns pulse duration, an improvement in the axial resolution of ~50% is demonstrated by using excitations with pulses of duration <100 ps. Details of an optoacoustic microscopy instrument, operating at 532 nm, capable to provide high-resolution axial and lateral optoacoustic images, are also presented. The capabilities of the instrument are demonstrated by in-vivo images of Xenopus laevis brain with a similar ~ 3.8 µm lateral resolution throughout the whole axial imaging range.

Original languageEnglish
Title of host publicationProceedings of SPIE
EditorsChulhong Kim, Jan Laufer, Vasilis Ntziachristos, Roger J. Zemp
Number of pages3
PublisherSPIE - International Society for Optical Engineering
Publication date2023
Article number1263106
ISBN (Electronic)9781510664715
DOIs
Publication statusPublished - 2023
EventOpto-Acoustic Methods and Applications in Biophotonics VI 2023 - Munich, Germany
Duration: 25 Jun 202326 Jun 2023

Conference

ConferenceOpto-Acoustic Methods and Applications in Biophotonics VI 2023
Country/TerritoryGermany
CityMunich
Period25/06/202326/06/2023
SeriesProceedings of SPIE - The International Society for Optical Engineering
Volume12631
ISSN0277-786X

Keywords

  • Axial resolution
  • Optoacoustic Imaging
  • Pico-second lasers

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