Enhancing RGI lyase thermostability by targeted single point mutations

Inês R. Silva, Dorte Møller Larsen, Carsten Jers, Patrick Derkx, Anne S. Meyer, Jørn Dalgaard Mikkelsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Rhamnogalacturonan I lyase (RGI lyase) (EC 4.2.2.-) catalyzes the cleavage of rhamnogalacturonan I in pectins by β-elimination. In this study the thermal stability of a RGI lyase (PL 11) originating from Bacillus licheniformis DSM 13/ATCC14580 was increased by a targeted protein engineering approach involving single amino acid substitution. Nine individual amino acids were selected as targets for site-saturated mutagenesis by the use of a predictive consensus approach in combination with prediction of protein mutant stability changes and B-factor iteration testing. After extensive experimental verification of the thermal stability of the designed mutants versus the original wild-type RGI lyase, several promising single point mutations were obtained, particularly in position Glu434 on the surface of the enzyme protein. The best mutant, Glu434Leu, produced a half-life of 31 min at 60 °C, corresponding to a 1.6-fold improvement of the thermal stability compared to the original RGI lyase. Gly55Val was the second best mutation with a thermostability half-life increase of 27 min at 60 °C, and the best mutations following were Glu434Trp, Glu434Phe, and Glu434Tyr, respectively. The data verify the applicability of a combinatorial predictive approach for designing a small site saturation library for improving enzyme thermostability. In addition, new thermostable RGI lyases suitable for enzymatic upgrading of pectinaceous plant biomass materials at elevated temperatures were produced.
Original languageEnglish
JournalApplied Microbiology and Biotechnology
Volume97
Issue number22
Pages (from-to)9727-9735
ISSN0175-7598
DOIs
Publication statusPublished - 2013

Keywords

  • Protein engineering
  • Semi-rational
  • Consensus approach
  • Bacillus licheniformis
  • Bacillus subtilis expression

Cite this