Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium

D.B. Muñiz-Marquez, J.C. Contreras, Raul Rodríguez , Solange Ines Mussatto, Jose A. Teixeira, C. N. Aguilar

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347U/L) was obtained at 32°C, using packing density of 0.7g/cm(3). Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.
Original languageEnglish
Title of host publicationInternational Conference on New Horizons in Biotechnology (NHBT-2015)
Volume213
PublisherElsevier
Publication date2016
Pages 276–282
DOIs
Publication statusPublished - 2016
Externally publishedYes
SeriesBioresource Technology
Volume213
ISSN0960-8524

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