Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium

D.B. Muñiz-Marquez; J.C. Contreras; Raul Rodríguez; Solange Ines Mussatto; Jose A.  Teixeira; C. N. Aguilar

doi:10.1016/j.biortech.2016.03.022

Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium

D.B. Muñiz-Marquez, J.C. Contreras, Raul Rodríguez , Solange Ines Mussatto, Jose A. Teixeira, C. N. Aguilar

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Abstract

The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347U/L) was obtained at 32°C, using packing density of 0.7g/cm(3). Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.

Original language	English
Title of host publication	International Conference on New Horizons in Biotechnology (NHBT-2015)
Volume	213
Publisher	Elsevier
Publication date	2016
Pages	276–282
DOIs	https://doi.org/10.1016/j.biortech.2016.03.022
Publication status	Published - 2016
Externally published	Yes

Series	Bioresource Technology
Volume	213
ISSN	0960-8524

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Muñiz-Marquez, D. B., Contreras, J. C., Rodríguez , R., Mussatto, S. I., Teixeira, J. A., & Aguilar, C. N. (2016). Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium. In International Conference on New Horizons in Biotechnology (NHBT-2015) (Vol. 213, pp. 276–282). Elsevier. https://doi.org/10.1016/j.biortech.2016.03.022

Muñiz-Marquez, D.B. ; Contreras, J.C. ; Rodríguez , Raul et al. / Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium. International Conference on New Horizons in Biotechnology (NHBT-2015). Vol. 213 Elsevier, 2016. pp. 276–282 (Bioresource Technology, Vol. 213).

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title = "Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium",

abstract = "The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347U/L) was obtained at 32°C, using packing density of 0.7g/cm(3). Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate. ",

author = "D.B. Mu{\~n}iz-Marquez and J.C. Contreras and Raul Rodr{\'i}guez and Mussatto, {Solange Ines} and Teixeira, {Jose A.} and Aguilar, {C. N.}",

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Muñiz-Marquez, DB, Contreras, JC, Rodríguez , R, Mussatto, SI, Teixeira, JA & Aguilar, CN 2016, Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium. in International Conference on New Horizons in Biotechnology (NHBT-2015). vol. 213, Elsevier, Bioresource Technology, vol. 213, pp. 276–282. https://doi.org/10.1016/j.biortech.2016.03.022

Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium. / Muñiz-Marquez, D.B.; Contreras, J.C.; Rodríguez , Raul et al.
International Conference on New Horizons in Biotechnology (NHBT-2015). Vol. 213 Elsevier, 2016. p. 276–282 (Bioresource Technology, Vol. 213).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

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AU - Muñiz-Marquez, D.B.

AU - Contreras, J.C.

AU - Rodríguez , Raul

AU - Mussatto, Solange Ines

AU - Teixeira, Jose A.

AU - Aguilar, C. N.

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AB - The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347U/L) was obtained at 32°C, using packing density of 0.7g/cm(3). Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.

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Muñiz-Marquez DB, Contreras JC, Rodríguez R, Mussatto SI, Teixeira JA, Aguilar CN. Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium. In International Conference on New Horizons in Biotechnology (NHBT-2015). Vol. 213. Elsevier. 2016. p. 276–282. (Bioresource Technology, Vol. 213). doi: 10.1016/j.biortech.2016.03.022

Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium

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