Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources

Harry R. Beller, Peng Zhou, Talia N.M. Jewell, Ee-Been Goh, Jay Keasling

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Abstract

Chemolithoautotrophic bacteria that oxidize reduced sulfur compounds, such as H2S, while fixing CO2 are an untapped source of renewable bioproducts from sulfide-laden waste, such as municipal wastewater. In this study, we report engineering of the chemolithoautotrophic bacterium Thiobacillus denitrificans to
produce up to 52-fold more fatty acids than the wild-type strain when grown with thiosulfate and CO2. A modified thioesterase gene from E. coli (‘tesA) was integrated into the T. denitrificans chromosome under the control of Pkan or one of two native T. denitrificans promoters. The relative strength of the two native
promoters as assessed by fatty acid production in engineered strains was very similar to that assessed by expression of the cognate genes in the wild-type strain. This proof-of-principle study suggests that engineering sulfide-oxidizing chemolithoautotrophic bacteria to overproduce fatty acid-derived products merits consideration as a technology that could simultaneously produce renewable fuels/chemicals as well as cost-effectively remediate sulfide-contaminated wastewater.
Original languageEnglish
JournalMetabolic Engineering Communications
Volume3
Pages (from-to)211-215
Number of pages5
ISSN2214-0301
DOIs
Publication statusPublished - 2016

Bibliographical note

© 2016 International Metabolic Engineering Society. Published by Elsevier B.V. International Metabolic Engineering Society. All rights reserved.

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