TY - JOUR
T1 - Enhanced enzymatic hydrolysis of rice straw by removal of phenolic compounds using a novel laccase from yeast Yarrowia lipolytica.
AU - Lee, Kyoung-Mi
AU - Kalyani, Dayanand
AU - Tiwari, Manish Kumar
AU - Kim, Tae-Su
AU - Dhiman, Saurabh Sudha
AU - Lee, Jung-Kul
AU - Kim, In-Won
PY - 2012
Y1 - 2012
N2 - Abstract: An extracellular laccase-producing yeast was isolated from soil and identified as Yarrowia lipolytica by its morphology and by comparison of its internal transcribed spacer rDNA gene sequence. Extracellular laccase (YlLac) from Y. lipolytica was purified to homogeneity by anion-exchange and gel filtration chromatography. YlLac is a monomeric glycoprotein with 14% carbohydrate content and a molecular mass of 67kDa. It showed a higher catalytic efficiency towards 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (k cat/K m =19.3s−1 μM−1) and 2,6-dimethoxyphenol (k cat/K m =13s−1 μM−1) than any other reported laccase. This enzyme was able to oxidize phenolic compounds present in pretreated rice straw. Several parameters (temperature, enzyme concentration, and mediator compounds) to enhance removal of phenolic compounds from pretreated rice straw were optimized using response surface methodology. The use of YlLac for the removal of cellulase inhibitory compounds from biomass slurries was found to be a promising approach for improving the efficiency of biorefineries.
AB - Abstract: An extracellular laccase-producing yeast was isolated from soil and identified as Yarrowia lipolytica by its morphology and by comparison of its internal transcribed spacer rDNA gene sequence. Extracellular laccase (YlLac) from Y. lipolytica was purified to homogeneity by anion-exchange and gel filtration chromatography. YlLac is a monomeric glycoprotein with 14% carbohydrate content and a molecular mass of 67kDa. It showed a higher catalytic efficiency towards 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (k cat/K m =19.3s−1 μM−1) and 2,6-dimethoxyphenol (k cat/K m =13s−1 μM−1) than any other reported laccase. This enzyme was able to oxidize phenolic compounds present in pretreated rice straw. Several parameters (temperature, enzyme concentration, and mediator compounds) to enhance removal of phenolic compounds from pretreated rice straw were optimized using response surface methodology. The use of YlLac for the removal of cellulase inhibitory compounds from biomass slurries was found to be a promising approach for improving the efficiency of biorefineries.
KW - ENZYMATIC analysis
U2 - 10.1016/j.biortech.2012.07.066
DO - 10.1016/j.biortech.2012.07.066
M3 - Journal article
VL - 123
SP - 636
EP - 645
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -