Enhanced enzymatic hydrolysis of rice straw by removal of phenolic compounds using a novel laccase from yeast Yarrowia lipolytica.

Kyoung-Mi Lee, Dayanand Kalyani, Manish Kumar Tiwari, Tae-Su Kim, Saurabh Sudha Dhiman, Jung-Kul Lee, In-Won Kim

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Abstract: An extracellular laccase-producing yeast was isolated from soil and identified as Yarrowia lipolytica by its morphology and by comparison of its internal transcribed spacer rDNA gene sequence. Extracellular laccase (YlLac) from Y. lipolytica was purified to homogeneity by anion-exchange and gel filtration chromatography. YlLac is a monomeric glycoprotein with 14% carbohydrate content and a molecular mass of 67kDa. It showed a higher catalytic efficiency towards 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (k cat/K m =19.3s−1 μM−1) and 2,6-dimethoxyphenol (k cat/K m =13s−1 μM−1) than any other reported laccase. This enzyme was able to oxidize phenolic compounds present in pretreated rice straw. Several parameters (temperature, enzyme concentration, and mediator compounds) to enhance removal of phenolic compounds from pretreated rice straw were optimized using response surface methodology. The use of YlLac for the removal of cellulase inhibitory compounds from biomass slurries was found to be a promising approach for improving the efficiency of biorefineries.
Original languageEnglish
JournalBioresource Technology
Volume123
Pages (from-to)636-645
ISSN0960-8524
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • ENZYMATIC analysis

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