Enhanced drug delivery to melanoma cells using PMPC-PDPA polymersomes

Carla Pegoraro; Denis Cecchin; Lorena Simon Gracia; Nicholas J. Warren; Peter Jeppe Madsen; Steven P. Armes; Andrew Lewis; Sheila MacNeil; Giuseppe Battaglia

doi:10.1016/j.canlet.2013.02.007

Enhanced drug delivery to melanoma cells using PMPC-PDPA polymersomes

Carla Pegoraro, Denis Cecchin, Lorena Simon Gracia, Nicholas J. Warren, Peter Jeppe Madsen, Steven P. Armes, Andrew Lewis, Sheila MacNeil, Giuseppe Battaglia

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We present the efficient and stable encapsulation of doxorubicin within pH sensitive polymeric vesicles (polymersomes) for intracellular and nuclear delivery to melanoma cells. We demonstrate that PMPC_25-PDPA₇₀ polymersomes can encapsulate doxorubicin for long periods of time without significant drug release. We demonstrate that empty polymersomes are non-toxic and that they are quickly and more efficiently internalised by melanoma cells compared to healthy cells. Encapsulated doxorubicin has a strong cytotoxic effect on both healthy and cancerous cells, but when encapsulated it had a preferential effect on melanoma cells indicating that this formulation can be used to achieve an enhanced drug delivery to cancerous cells rather than to the healthy surrounding cells.

Original language	English
Journal	Cancer Letters
Volume	334
Issue number	2
Pages (from-to)	328-337
Number of pages	10
ISSN	0304-3835
DOIs	https://doi.org/10.1016/j.canlet.2013.02.007
Publication status	Published - 2013
Externally published	Yes

Keywords

Polymersomes
Melanoma
Targeted delivery
Doxorubicin

Access to Document

10.1016/j.canlet.2013.02.007

Cite this

Pegoraro, C., Cecchin, D., Gracia, L. S., Warren, N. J., Jeppe Madsen, P., Armes, S. P., Lewis, A., MacNeil, S., & Battaglia, G. (2013). Enhanced drug delivery to melanoma cells using PMPC-PDPA polymersomes. Cancer Letters, 334(2), 328-337. https://doi.org/10.1016/j.canlet.2013.02.007

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title = "Enhanced drug delivery to melanoma cells using PMPC-PDPA polymersomes",

abstract = "We present the efficient and stable encapsulation of doxorubicin within pH sensitive polymeric vesicles (polymersomes) for intracellular and nuclear delivery to melanoma cells. We demonstrate that PMPC25-PDPA70 polymersomes can encapsulate doxorubicin for long periods of time without significant drug release. We demonstrate that empty polymersomes are non-toxic and that they are quickly and more efficiently internalised by melanoma cells compared to healthy cells. Encapsulated doxorubicin has a strong cytotoxic effect on both healthy and cancerous cells, but when encapsulated it had a preferential effect on melanoma cells indicating that this formulation can be used to achieve an enhanced drug delivery to cancerous cells rather than to the healthy surrounding cells.",

keywords = "Polymersomes, Melanoma, Targeted delivery, Doxorubicin",

author = "Carla Pegoraro and Denis Cecchin and Gracia, {Lorena Simon} and Warren, {Nicholas J.} and {Jeppe Madsen}, Peter and Armes, {Steven P.} and Andrew Lewis and Sheila MacNeil and Giuseppe Battaglia",

year = "2013",

doi = "10.1016/j.canlet.2013.02.007",

language = "English",

volume = "334",

pages = "328--337",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier",

number = "2",

}

Enhanced drug delivery to melanoma cells using PMPC-PDPA polymersomes. / Pegoraro, Carla; Cecchin, Denis; Gracia, Lorena Simon; Warren, Nicholas J.; Jeppe Madsen, Peter; Armes, Steven P.; Lewis, Andrew; MacNeil, Sheila; Battaglia, Giuseppe.

In: Cancer Letters, Vol. 334, No. 2, 2013, p. 328-337.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Enhanced drug delivery to melanoma cells using PMPC-PDPA polymersomes

AU - Pegoraro, Carla

AU - Cecchin, Denis

AU - Gracia, Lorena Simon

AU - Warren, Nicholas J.

AU - Jeppe Madsen, Peter

AU - Armes, Steven P.

AU - Lewis, Andrew

AU - MacNeil, Sheila

AU - Battaglia, Giuseppe

PY - 2013

Y1 - 2013

N2 - We present the efficient and stable encapsulation of doxorubicin within pH sensitive polymeric vesicles (polymersomes) for intracellular and nuclear delivery to melanoma cells. We demonstrate that PMPC25-PDPA70 polymersomes can encapsulate doxorubicin for long periods of time without significant drug release. We demonstrate that empty polymersomes are non-toxic and that they are quickly and more efficiently internalised by melanoma cells compared to healthy cells. Encapsulated doxorubicin has a strong cytotoxic effect on both healthy and cancerous cells, but when encapsulated it had a preferential effect on melanoma cells indicating that this formulation can be used to achieve an enhanced drug delivery to cancerous cells rather than to the healthy surrounding cells.

AB - We present the efficient and stable encapsulation of doxorubicin within pH sensitive polymeric vesicles (polymersomes) for intracellular and nuclear delivery to melanoma cells. We demonstrate that PMPC25-PDPA70 polymersomes can encapsulate doxorubicin for long periods of time without significant drug release. We demonstrate that empty polymersomes are non-toxic and that they are quickly and more efficiently internalised by melanoma cells compared to healthy cells. Encapsulated doxorubicin has a strong cytotoxic effect on both healthy and cancerous cells, but when encapsulated it had a preferential effect on melanoma cells indicating that this formulation can be used to achieve an enhanced drug delivery to cancerous cells rather than to the healthy surrounding cells.

KW - Polymersomes

KW - Melanoma

KW - Targeted delivery

KW - Doxorubicin

U2 - 10.1016/j.canlet.2013.02.007

DO - 10.1016/j.canlet.2013.02.007

M3 - Journal article

VL - 334

SP - 328

EP - 337

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 2

ER -