Enhanced biodegradation by hydraulic heterogeneities in petroleum hydrocarbon plumes

Robert D. Bauer, Massimo Rolle, Sebastian Bauer, Christina Eberhardt, Peter Grathwohl, Olaf Kolditz, Rainer U. Meckenstock, Christian Griebler

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In case of dissolved electron donors and acceptors, natural attenuation of organic contaminant plumes in aquifers is governed by hydrodynamic mixing and microbial activity. Main objectives of this work were (i) to determine whether aerobic and anaerobic biodegradation in porous sediments is controlled by transverse dispersion, (ii) to elucidate the effect of sediment heterogeneity on mixing and biodegradation, and (iii) to search for degradation-limiting factors. Comparative experiments were conducted in two-dimensional sediment microcosms. Aerobic toluene and later ethylbenzene degradation by Pseudomonas putida strain F1 was initially followed in a plume developing from oxic to anoxic conditions and later under steady-state mixing-controlled conditions. Competitive anaerobic degradation was then initiated by introduction of the denitrifying strain Aromatoleum aromaticum EbN1. In homogeneous sand, aerobic toluene degradation was clearly controlled by dispersive mixing. Similarly, under denitrifying conditions, microbial activity was located at the plume's fringes. Sediment heterogeneity caused flow focusing and improved the mixing of reactants. Independent from the electron accepting process, net biodegradation was always higher in the heterogeneous setting with a calculated efficiency plus of 23-100% as compared to the homogeneous setup. Flow and reactive transport model simulations were performed in order to interpret and evaluate the experimental results. © 2008 Elsevier B.V. All rights reserved.
Original languageEnglish
JournalJournal of Contaminant Hydrology
Volume105
Issue number1-2
Pages (from-to)56-68
Number of pages13
ISSN0169-7722
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Water Science and Technology
  • Environmental Chemistry
  • Biodegradation
  • Contaminant plume
  • Natural attenuation
  • Sediment heterogeneity
  • Toluene oxidation
  • Transverse dispersion
  • Aquifers
  • Biochemistry
  • Degradation
  • Denitrification
  • Dissolution
  • Ethylbenzene
  • Fluid dynamics
  • Fullerenes
  • Groundwater pollution
  • Hydrocarbons
  • Hydrogeology
  • Microbiology
  • Oxidation
  • Petroleum chemistry
  • Sedimentology
  • Sediments
  • Toluene
  • anoxic conditions
  • bacterium
  • benzene
  • biodegradation
  • comparative study
  • denitrification
  • electron
  • experimental study
  • heterogeneity
  • microbial activity
  • microcosm
  • natural attenuation
  • organic pollutant
  • oxidation
  • petroleum
  • toluene
  • Anaerobiosis
  • Biodegradation, Environmental
  • Computer Simulation
  • Electrons
  • Oxygen
  • Petroleum
  • Soil Pollutants
  • Water Microbiology
  • Pseudomonas putida

Cite this

Bauer, R. D., Rolle, M., Bauer, S., Eberhardt, C., Grathwohl, P., Kolditz, O., Meckenstock, R. U., & Griebler, C. (2009). Enhanced biodegradation by hydraulic heterogeneities in petroleum hydrocarbon plumes. Journal of Contaminant Hydrology, 105(1-2), 56-68. https://doi.org/10.1016/j.jconhyd.2008.11.004